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Selenotest ELISA kit说明书

更新时间：2016-10-25 点击次数：1870

immunochemical inligence GmbH?是现在*生产selenoprotein P?试剂盒的厂家，该试剂盒是使用ELISA方法来测定人血浆中selenoprotein P蛋白含量，方便检测

selenoprotein P (SEPP1)研究背景

亚细胞定位: Secreted.

组织特异性: Made in the liver and heart and secreted into the plasma. It is also found in the kidney.

翻译后修饰: Phosphorylation sites are present in the extracellular medium.

相似的序列: Belongs to the selenoprotein P family.

品牌	immunochemical inligence GmbH
产品货号	STE
产品名称	Selenotest ELISA kit
用途	检测人类血浆selenoprotein P (SEPP1)蛋白含量
包装	96孔板
检测方法	比色法 Colorimetric (450 nm)
样品需要量	5 – 10 µL
采样类型	血浆
检测时间	5 – 6小时
灵敏度	‹ 8 µg/L (about 500-fold below the serum level of healthy individuals)
检测范围	Approx. 5 – 800 µg/L
定量范围	Approx. 10 – 400 µg/L (corresponds to 0.2 – 8 mg/L in undiluted sample)
试剂盒保存温度	Refrigerated (2 – 8度)
保质期	6个月到中国客户手上至少3个月
试剂盒尺寸(L×W×H)	200 × 150 × 95毫米
试剂盒重量	400g
生产地址	德国
价格	750欧元，7500RMB
到中国运输费用	2000元
到中国的时间	4周左右
代理商	上海起发实验试剂有限公司
关键词	SELENOP selenoprotein P SEPP1
成分组成：
包被好的96孔板	1：Pre-coated, pre-blocked 96 well microplate (1 plate, ready-for-use)
冻干血浆标准品 8管	2：Serum Calibrators, lyophilized (8 tubes, 0.5 mL each)
阳性对照 3管	3：Serum Controls, lyophilized (3 tubes, 0.5 mL each)
阴性对照 1管	4：Assay Blank, lyophilized (1 tube, 0.5 mL)
重建缓冲液 8ML 一瓶	5：Reconstitution Buffer (1 bottle, 8 mL)
样品稀释液20ml一瓶	6：Sample Dilution Buffer (1 bottle, 20 mL)
生物素标记抗体125ul	7:Concentrated biotin-conjugated Detection Antibody (DAB), liquid (1 tube, 0.125 mL)
链霉亲和素过氧化物酶复合物	8:Concentrated Streptavidin-Peroxidase Complex (POD), liquid (1 tube, 0.125 mL)
DAB稀释液11ML	9:DAB Dilution Buffer (1 bottle, 11 mL)
POD稀释液11ml	10:POD Dilution Buffer (1 bottle, 11 mL)
20x洗涤缓冲液25ml	11:Washing Buffer, 20× concentrate (1 bottle, 25 mL)
POD底物溶液11ml	12:POD Substrate Solution (1 bottle, 11 mL)
终止液11ml	13:Stop Solution (1 bottle, 11 mL)
封板膜4张	14:Plate Cover Foil (4 pieces)
操作指南一份英文	15:Instructions For Use
产品清单一份	16:Product Specification Sheet
质量检查报告一份	17:Quality Control Certificate

需要额外准备的产品
移液器及配套吸头	Calibrated, adjustable precision single- and multichannel pipettes with disposable plastic tips
各种样品管或者微孔板	Polypropylene 96 well microplate or tubes for diluting samples
混匀器	Vortex mixer
离心机	Centrifuge for microtubes
洗板机	Automated microplate washer (option)
摇床	Microplate shaker
分光光度计	Microplate reader capable measuring absorbance at 450 nm
吸水纸	Absorbent paper towels
纯净水	Laboratory water (type 1 or 2)
相关软件	Data analysis and graphing software

References（引用文献）

[1] Hybsier S, Wu Z, Schulz T, Strasburger CJ, Köhrle J, Minich WB, Schomburg L. Establishment and characterization of a new ELISA for selenoprotein P. Perspectives in Science. 2015 Feb;3(1-4):23-4. http://dx.doi.org/10.1016/j.pisc.2014.11.013

[2] Ballihaut G, Kilpatrick LE, Kilpatrick EL, Davis WC. Multiple forms of selenoprotein P in a candidate human plasma standard reference material. Metallomics. 2012 Jun;4(6):533-8. http://dx.doi.org/10.1039/c2mt20059g

[3] Ballihaut G, Kilpatrick LE, Davis WC. Detection, identification, and quantification of selenoproteins in a candidate human plasma standard reference material. Anal Chem. 2011 Nov;83(22):8667-74. http://dx.doi.org/10.1021/ac2021147

资料下载：

Poster Presentations

The Selenotest ELISA was first presented at the GMS Jahrestagung 2013 (Gesellschaft für Mineralstoffe und Spurenelemente) and at the 10th International Symposium on Selenium in Biology and Medicine 2013 in Berlin, Germany:

[pdf, 199KB] Hybsier S, Wu Z, Schulz T, Strasburger CJ, Köhrle J, Minich WB, Schomburg L. Establishment and characterization of a new ELISA for selenoprotein P. Poster Presentation at 10^thInternational Symposium on Selenium in Biology and Medicine 2013, Berlin.

Instructions For Use

[pdf] Arbeitsanleitung (Version 3, DE) [pdf] Instructions for use (Version 3, EN)

Product Specification Sheets

[pdf] Lot:MSP013001 [pdf] Lot:MSP013002 [pdf] Lot:MSP014001 [pdf] Lot:MSP015001

Quality Control (QC) Certificates

[pdf] Lot:MSP013001 [pdf] Lot:MSP013002 [pdf] Lot:MSP014001 [pdf] Lot:MSP015001

Material safety data sheets

[pdf] Stop Solution (STO)