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α-凝血酶α-Thrombin选购指南

更新时间：2016-11-01 点击次数：4105

凝血酶Thrombin (Factor IIa)是一种丝氨酸蛋白酶，也是血液凝血级联反应中的主要效应蛋白酶，显现出促凝和抗凝的特性。凝血酶是由在凝血酶原复合物中的非活性凝血酶原在Xa因子（FXa）因子的作用下通过蛋白裂解的方式产生

【生理作用】当循环凝血因子在暴露的血管外组织与组织因子相接触时，凝血酶会在组织上聚集。凝血酶通过激活血小板，催化纤维蛋白原转化为纤维蛋白，促进血稳定而在血栓性疾病的引发和传播上有着核心作用

α-凝血酶（α-Thrombin）是一种高度特异性的丝氨酸蛋白酶，由凝血酶原（prothrombin，II因子）经蛋白水解活化而来。它在凝血过程中起着非常重要的作用，不仅能够促使纤维蛋白凝块的产生，还负责提供反馈信号来激活前辅因子：V因子和VIII因子。也可以激活XIII因子和血小板，或者用作血管收缩剂。

在体内，活化的X因子（Factor Xa）切割凝血酶原，释放出活性肽并将凝血酶切割成具有催化活性的α-凝血酶。α-凝血酶由一条轻链（A chain）（Mw ~6,000）和一条重链（B chain）（Mw ~31,000）通过二硫键连接而成。某些情况下，α-凝血酶会发生自溶生成β-凝血酶和γ-凝血酶。β-凝血酶由对α-凝血酶A链水解并切割含有B链糖基化位点的小片段（B1，B2）组合而成。

除了用于凝血研究之外，α-凝血酶还常用来位点特异性切割融合蛋白。基因重组时将凝血酶识别位点插入在目的蛋白与利于后续纯化和/或表达的多肽或者蛋白之间，通过凝血酶切割表达的重组子即可释放目的蛋白。凝血酶本身可通过亲和层析技术快速去除。

作为生物活性物质的凝血酶：是一种在人体抗凝，促凝的凝血级联反应中有着重要作用的生物活性蛋白。

产品性质

中文别名（Chinese synonym）	α-凝血酶；IIa 因子；
英文别名（English synonym）	α-Thrombin; Factor IIa;
CAS号（CAS NO.）	9002-04-4
分子量（Molecular weight）	37000 daltons
活力（Activity）	≥2700.00 NIH U/mg
缓冲液组分（Buffer）	50mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5
消光系数（Extinction coefficient）（1%）	18.3

运输和保存方法

粉末冰袋运输，2-8℃保存。液体干冰运输，≤-60℃保存。

凝血酶Thrombin (Factor IIa) 抗体

11571-05021	Thrombin, anti-human	150 ug	2800	AssayPro
11571-05011	Thrombin, anti-human	150 ug	2320	AssayPro
BP104C	Bovine anti-Thrombin (AT)	1 x 10 mg	4545	hyphen biomed
60130-05021	alpha-Thrombin (Factor IIa), anti-human	150 ug	2320	AssayPro
60130-05011	alpha-Thrombin (Factor IIa), anti-human	150 ug	2000	AssayPro
SAHT-AP	Anti-human Thrombin (F.IIa) sheep, AP IgG,	0.5 mg	5168	Enzyme Research2016
PAHT-S	Sheep anti-Human Thrombin	1x1 mg	436.8	Haematologic Technologies
PAHT-S	Sheep anti-Human Thrombin	1x5 mg	1506.96	Haematologic Technologies
SART-IG	Sheep anti rabbit Thrombin purified IgG	10 mg	2414	affinitybiologicals
SART-HRP	Sheep anti rabbit Thrombin peroxidase conjugated IgG	0.2 mg	4131	affinitybiologicals
SART-AP	Sheep anti rabbit Thrombin affinity purified IgG	0.5 mg	5304	affinitybiologicals
SAHT-IG	Sheep anti human Thrombin purified IgG	10 mg	2227	affinitybiologicals
SAHT-HRP	Sheep anti human Thrombin peroxidase conjugated IgG	0.2 mg	4029	affinitybiologicals
SAHT-APFTC	Sheep anti human Thrombin affinity purified, FITC conjugated IgG	0.1 mg	4029	affinitybiologicals
SAHT-APBIO	Sheep anti human Thrombin affinity purified, Biotinylated IgG	0.1 mg	4029	affinitybiologicals
SAHT-AP	Sheep anti human Thrombin affinity purified IgG	0.5 mg	5168	affinitybiologicals
SABT-IG	Sheep anti bovine Thrombin purified IgG	10 mg	2227	affinitybiologicals
SABT-HRP	Sheep anti bovine Thrombin peroxidase conjugated IgG	0.2 mg	4029	affinitybiologicals
SABT-AP	Sheep anti bovine Thrombin affinity purified IgG	0.5 mg	5168	affinitybiologicals
SART-IG	Anti-rabbit Thrombin (rF.IIa)sheep, purified IgG, 10 mg	10 mg	2600	Enzyme Research2016
SART-HRP	Anti-rabbit Thrombin (rF.IIa)sheep, perox-conj. IgG,	0.2 mg	4131	Enzyme Research2016
SART-AP	Anti-rabbit Thrombin (rF.IIa)sheep, affinity purified IgG,	0.5 mg	5304	Enzyme Research2016
SATAFI-AP	Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, affinity purified IgG,	0.5 mg	5066	Enzyme Research2016
SAHT-HRP	Anti-human Thrombin (F.IIa)sheep, perox-conj. IgG,	0.2 mg	4029	Enzyme Research2016
SAHT-IG	Anti-human Thrombin (F.IIa) sheep, purified IgG,	10 mg	2600	Enzyme Research2016
SAHT-APFTC	Anti-human Thrombin (F.IIa) sheep, AP-FITC	0.1 mg	4029	Enzyme Research2016
AHT-5020	Anti-Human Thrombin	1x100 µg	1179.36	Haematologic Technologies
AHT-5020	Anti-Human Thrombin	5x100 µg	3057.6	Haematologic Technologies
SABT-IG	Anti-bovine Thrombin (bF.IIa)sheep, purified IgG,	10 mg	2600	Enzyme Research2016
SABT-HRP	Anti-bovine Thrombin (bF.IIa)sheep, perox-conj. IgG,	0.2 mg	4029	Enzyme Research2016
SABT-AP	Anti-bovine Thrombin (bF.IIa)sheep, affinity purified IgG,	0.5 mg	5168	Enzyme Research2016
SATAFI-IG	Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, purified IgG,	10 mg	2635	Enzyme Research2016

凝血酶Thrombin (Factor IIa) 试剂盒

货号	品名	包装	价格	品牌
ET4010-1	Human Thrombin ELISA Kit	96 well plate	4760	AssayPro
Thrombin-HCII Complex	Thrombin-HCII Complex elisa kit	5 plate	8024	Enzyme Research2016
ADG844	OLIGOBIND® Thrombin activity assay	96 microwell plate	9200	American Diagnostica Inc

凝血酶Thrombin (Factor IIa) 抑制剂

货号	品名	包装	价格	品牌
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	1x50 µg	4298.83272	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	1x1 mg	68592.36384	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	5x50 µg	19250.2674	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	5x1 mg	308594.7774	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	10x1 mg	555305.2596	Haematologic Technologies
CK002L	HEMOCLOT Thrombin Inhibitors	3x2.5 mL	2130	hyphen biomed
CK002K	HEMOCLOT Thrombin Inhibitors	3x1 mL	1110	hyphen biomed

凝血酶Thrombin (Factor IIa) 发色底物

SN-17a	D-FPR-ANSNH-C6H11 • 2 HCl	100x1 mg	184235.688	Haematologic Technologies
SN-59	D-VPR-ANSNH-C4H9 • 2 HCl	1x1 mg	1842.35688	Haematologic Technologies
229005	BIOPHEN CS-01(81) - Thrombin Chromogenic Substrate	1 x 25 mg	3000	hyphen biomed
229001	BIOPHEN CS-01(38) - Thrombin Chromogenic substrate	1 x 25 mg	3000	hyphen biomed
0082032439	S-2238 H-D-Phe-Pip-Arg-pNA•2HCl	25mg	3500	Chromogenix2014
SN-20	Boc-L-FPR-ANSNH-C2H5	1x1 mg	1842.35688	Haematologic Technologies

凝血酶Thrombin (Factor IIa) ?其他耗材和试剂

ADG844T50	Thrombin blood collection tubes, 3 ml	50 tubes	5525	American Diagnostica Inc
ADG844T25	Thrombin blood collection tubes, 3 ml	25 tubes	3025	American Diagnostica Inc
CK011L	HEMOCLOT Thrombin Time (T.T.)	6 x 8 mL	915	hyphen biomed
CK011K	HEMOCLOT Thrombin Time (T.T.)	6 x 2 mL	285	hyphen biomed
CK001L	HEMOCLOT Thrombin (human)	6 x 8 mL	1155	hyphen biomed
CK001K	HEMOCLOT Thrombin (human)	6 x 2 mL	420	hyphen biomed

凝血酶Thrombin (Factor IIa) 蛋白

货号	品名	包装	价格	品牌
PT62082	Thrombin, Mouse	50 ug	3900	AssayPro
PP004K	Anti-Thrombin (AT)	6 x 150 µg	3030	hyphen biomed
PP004B	Anti-Thrombin (AT)	1 x 1.5 mg	1545	hyphen biomed
PP004A	Anti-Thrombin (AT)	1 x 150 µg	720	hyphen biomed
HT 1002a	Human a-Thrombin Priced per 1000 units	3,000+ units	2600	Enzyme Research2016
HT 1002a	Human a-Thrombin Priced per 1000 units	<3,000 units	2600	Enzyme Research2016
BT 1002a	Bovine Thrombin (Priced per 1000 units)	<3,000 units	2600	Enzyme Research2016
RIIa	Rat Thrombin	0.1 mg	5199.9192	Enzyme Research2016
RbIIa	Rabbit Thrombin	0.1 mg	5199.9192	Enzyme Research2016
PIIa	Porcine Thrombin	0.1 mg	5199.9192	Enzyme Research2016
MIIa	Murine Thrombin	0.05 mg	2600	Enzyme Research2016
MCT-5020	Mouse Thrombin	1x50 µg	4004.1456	Haematologic Technologies
MCT-5020	Mouse Thrombin	5x50 µg	17771.208	Haematologic Technologies
MCT-5020	Mouse Thrombin	1x1 mg	63116.34	Haematologic Technologies
MCT-5020	Mouse Thrombin	25x1 mg	1277277.456	Haematologic Technologies
MCT-5020	Mouse Thrombin	5x1 mg	283889.424	Haematologic Technologies
HTi	Human Thrombin Inactivated	1.0 mg	2600	Enzyme Research2016
EZ006O	Human Thrombin	1 x 10 NIH	195	hyphen biomed
EZ006K	Human Thrombin	6 x 10 NIH	825	hyphen biomed
EZ006B	Human Thrombin	1 x 1000 NIH	3765	hyphen biomed
HCGT-0021	Human gamma-thrombin	1x100 µg	992.03832	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	5x100 µg	4487.7924	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	5x1 mg	38736.7344	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	10x1 mg	76292.4708	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	50x1 mg	343670.418	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	100x1 mg	670806.864	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	500x1 mg	3035164.86	Haematologic Technologies
HCBT-0022	Human beta-thrombin	1x100 µg	992.03832	Haematologic Technologies
HCBT-0022	Human beta-thrombin	5x100 µg	4487.7924	Haematologic Technologies
HCBT-0022	Human beta-thrombin	5x1 mg	38736.7344	Haematologic Technologies
HCBT-0022	Human beta-thrombin	10x1 mg	76292.4708	Haematologic Technologies
HCBT-0022	Human beta-thrombin	50x1 mg	343670.418	Haematologic Technologies
HCBT-0022	Human beta-thrombin	100x1 mg	670806.864	Haematologic Technologies
HCBT-0022	Human beta-thrombin	500x1 mg	3035164.86	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	1x100 µg	850.31856	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	1x1mg	5172.77124	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	5x100 µg	3542.994	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	5x1mg	25509.5568	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	10x1mg	46295.1216	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	50x1mg	226751.616	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	100x1mg	403901.316	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	1x100 µg	850.31856	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	1x1 mg	5172.77124	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	5x100 µg	3542.994	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	5x1 mg	25509.5568	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	10x1 mg	46295.1216	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	50x1 mg	226751.616	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	100x1 mg	403901.316	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	1x100 µg	850.31856	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	1x1 mg	5172.77124	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	5x100 µg	3542.994	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	5x1 mg	25509.5568	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	10x1 mg	46295.1216	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	50x1 mg	226751.616	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	100x1 mg	403901.316	Haematologic Technologies
HCT-0020	Human alpha-thrombin	1x100 µg	698.88	Haematologic Technologies
HCT-0020	Human alpha-thrombin	1x1 mg	2140.32	Haematologic Technologies
HCT-0020	Human alpha-thrombin	5x100 µg	2948.4	Haematologic Technologies
HCT-0020	Human alpha-thrombin	5x1 mg	9172.8	Haematologic Technologies
HCT-0020	Human alpha-thrombin	10x1 mg	16816.8	Haematologic Technologies
HCT-0020	Human alpha-thrombin	50x1 mg	81900	Haematologic Technologies
HCT-0020	Human alpha-thrombin	100x1 mg	146328	Haematologic Technologies
HCT-0020	Human alpha-thrombin	500x1 mg	677040	Haematologic Technologies
HGT	H. Gamma Thrombin	1.0 mg	6827.1048	Enzyme Research2016
CIIa	Canine Thrombin	0.1 mg	5199.9192	Enzyme Research2016
BT 1002a	Bovine Thrombin (Priced per 1000 units)	3,000+ units	2600	Enzyme Research2016
BE501K	Bovine Thrombin	10 x 500 NIH	14850	hyphen biomed
BE501C	Bovine Thrombin	1 x 500 NIH	1875	hyphen biomed
BE102K	Bovine Thrombin	6 x 21 NIH	885	hyphen biomed
BE102C	Bovine Thrombin	1 x 1000 NIH	3030	hyphen biomed
BE102B	Bovine Thrombin	1 x 100 NIH	735	hyphen biomed
BE102A	Bovine Thrombin	1 x 21 NIH	195	hyphen biomed
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	1x200 µg	992.03832	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	1x1 mg	3377.65428	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	5x200 µg	4015.3932	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	5x1 mg	16652.0718	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	10x1 mg	29761.1496	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	50x1 mg	145262.754	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	100x1 mg	266905.548	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	1x200 µg	992.03832	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	1x1 mg	3377.65428	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	5x200 µg	4015.3932	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	5x1 mg	16652.0718	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	10x1 mg	29761.1496	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	50x1 mg	145262.754	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	100x1 mg	266905.548	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	1x200 µg	992.03832	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	1x1 mg	3377.65428	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	5x200 µg	4015.3932	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	5x1 mg	16652.0718	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	10x1 mg	29761.1496	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	50x1 mg	145262.754	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	100x1 mg	266905.548	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	1x200 µg	786.24	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	1x1 mg	2140.32	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	5x200 µg	3494.4	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	5x1 mg	9172.8	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	10x1 mg	16816.8	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	50x1 mg	81900	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	100x1 mg	146328	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	500x1 mg	677040	Haematologic Technologies
0081079639	AT III 1x25IU	1x25IU	5500	Chromogenix2014
0082072039	AT III 10x10 IU	10x10 IU	14780	Chromogenix2014
470HT	alpha-thrombin, human	2,500 unit	5628	American Diagnostica Inc