上海起发Glycotope 10-0200大量现货

· Functional test ex vivo

来自体内的功能测试

· Evaluation of single cells to detect heterogenous populations

评价检测单个细胞异质种群

· Whole blood assay: No isolation procedures and optimal culture medium

全血检测：没有隔离程序和*培养基

· Physiological stimulans for phagocytes: Bacteria and fMLP

吞噬细胞生理兴奋劲：细菌和fMLP

· Dose response: Low and high stimulant

没有静电工件比较乳胶珠子聚苯乙烯管内

· Standardized test procedure

标准化测试程序

· Exclusion of aggregation artifacts by DNA staining

通过DNA染色排除聚合工件

· Compatible with whole blood of mice and rats

兼容的小鼠和大鼠全血

· Fast assay: Whole assay time is 1.5 hours

快速测定:整个试验时间是1.5小时

100 analyses. Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Blood. Evaluation by flow cytometry.

100次分析。临床诊断人体全血白细胞氧化破裂的定量检测。通过流式细胞术进行评估。

Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Bloods

SUMMARY and EXPLANATION

BURSTTEST (PHAGOBURST) allows the quantitative determination of leukocyte oxidative burst in heparinized whole blood. It contains unlabeled opsonized bacteria (E.coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP) as stimulants, Dihydrorhodamine (DHR) 123 as a fluorogenic substrate and necessary reagents. It determines the percentage of phagocytic cells which produce reactive oxidants (conversion of DHR 123 to R 123) and their enzymatic activity (amount of R 123 per cell).

The evaluation of these bioactivities should be performed by flow cytometry.

BURSTTEST(PHAGOBURST)允许定量测定白细胞氧化闯入肝素化全血。 它包含未标记的促进调理作用的细菌(大肠杆菌),佛波醇12十四烷酸乙酸13(PMA)和趋化作用的多肽n甲酰遇见亮氨酸板式换热器(fMLP),二氢若丹明(DHR)123作为一个荧光衬底和必要的试剂。它决定了吞噬细胞产生活性氧化剂的比例(转换DHR 123 到 R 1233)及其酶活性(每个细胞R 123的数量)。

这些生物活性的测定应该有流式细胞术完成。