Anti-TTF1 - Rabbit (WRAB-1231)使用说明
产品信息
品牌 | Seven Hills Bioreagents |
货号 | WRAB-1231 |
规格 | 100 µL |
宿主 | 兔 |
反应种属 | 人、鼠 |
克隆类型 | 多克隆 |
偶联物 | 无 |
缓冲液 | 0.01% sodium azide |
抗原 | 重组鼠TTF-1融合6*his蛋白 |
保存条件 | 储存在-20℃,避免反复冻融 |
蛋白背景 | 甲状腺转录因子-1(TTF-1)是同源域转录因子家族的成员,并且在调节甲状腺,肺和脑内表达的基因中起作用。 |
产品使用
实验应用 | 建议稀释倍数 |
Western Blot | 1: 5000 |
Immunohistochemistry | 1:1000 - 1:2000 |
说明:最佳稀释倍数与浓度应由研究人员确认
应用实例
图片为成年小鼠肺组织,用6%正常山羊血清预包埋,并用抗大鼠TTF-1兔血清(1:2000稀释)染色。通过DAB(黑色)观察TTF-1,并用核快红(粉红色)复染。放大倍数10倍。
参考文献
1. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
2. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8.
3. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700.
4. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
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