您好!欢迎访问上海起发实验试剂有限公司网站!
全国服务咨询热线:

15921799099

当前位置:首页 > 技术文章 > athensresearch 16-14-051200说明书——上海起发

athensresearch 16-14-051200说明书——上海起发

更新时间:2024-01-12      点击次数:197


Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。


athensresearch 16-14-051200说明书——上海起发


Elastase, Human Neutrophil


MW:29500

消光系数:0.985

无盐冻干固体。

重组:离心后,我们建议将50mM醋酸钠(pH 5.5)和150mM NaCl加入到原始体积中,然后轻轻混合以确保充分均质。

储存:<=-20°C

这种酶降解弹性蛋白、胶原蛋白和蛋白多糖,并与肺动脉塞和类风湿性关节炎的发展有关。

参考:Baugh,R.J.和Travis,J.1976。生物化学15,836;Bode,W.等人,1986年。EMBO J.5(10),2453

活性:每毫克蛋白质20-22个单位。一个单位被定义为在25摄氏度、pH 7.5下每分钟水解一摩尔MeO-Suc-Ala-Ala-Pro-Val-pNA的酶的量。

纯度:SDS-PAGE显示>=95%

由经美国食品药品监督管理局要求的检测显示对HBsAg、抗-HCV、抗-HBc无反应且抗-HIV 1和2呈阴性的全血制备。

雅典研究与技术公司的产品是实验室试剂,不得用于人体或任何药物用途



Kerros C, et al.
Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells.
J Biol Chem. 2017 Jun 16;292(24):10295-10305. doi: 10.1074/jbc.M116.773051. Epub 2017 May 3.

Martin KR, Kantari-Mimoun C, Yin M, Pederzoli-Ribeil M, et al.
Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.
J Biol Chem. 2016 May 13;291(20):10476-89. doi: 10.1074/jbc.M115.698639. Epub 2016 Mar 9.

Edwards JV, Fontenot KR, Prevost NT, Pircher N, Liebner F, Condon BD.
Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton.
Sensors (Basel). 2016 Oct 26;16(11). pii: E1789.

Chawla A, Alatrash G, Philips AV, Qiao N, et al.
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Cancer Immunol Immunother. 2016 Jun;65(6):741-51. doi: 10.1007/s00262-016-1841-6. Epub 2016 Apr 29.

Sinden NJ, et al.
α-1-Antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease.
Am J Physiol Lung Cell Mol Physiol. 2015 Jan 15;308(2):L179-90.

Ksiazek M, et al.
Miropin, a Novel Bacterial Serpin from the Periodontopathogen Tannerella forsythia, Inhibits a Broad Range of Proteases by Using Different Peptide Bonds within the Reactive Center Loop.
J Biol Chem. 2015 Jan 2;290(1):658-70. doi: 10.1074/jbc.M114.601716. Epub 2014 Nov 11.

Ramadass M, Ghebrehiwet B, Kew RR.
Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood.
Mol Immunol. 2015 Mar;64(1):55-62. doi: 10.1016/j.molimm.2014.10.022. Epub 2014 Nov 15.

Thomas MP, et al.
Leukocyte Protease Binding to Nucleic Acids Promotes Nuclear Localization and Cleavage of Nucleic Acid Binding Proteins.
J Immunol. 2014 Jun 1;192(11):5390-7. doi: 10.4049/jimmunol.1303296. Epub 2014 Apr 25.

Ungurs MJ, Sinden NJ, Stockley RA.
Progranulin is a substrate for neutrophil-elastase and proteinase-3 in the airway and its concentration correlates with mediators of airway inflammation in COPD
Am J Physiol Lung Cell Mol Physiol. 2014 Jan 1;306(1):L80-7. doi: 10.1152/ajplung.00221.2013. Epub 2013 Nov 1.

Sinden NJ, Koura F, Stockley RA.
The significance of the F variant of alpha-1-antitrypsin and unique case report of a PiFF homozygote.
BMC Pulm Med. 2014 Aug 7;14:132. doi: 10.1186/1471-2466-14-132.

Kenninston JA, et al.
Inhibition of Plasma Kallikrein by a Highly Specific Active Site Blocking Antibody.
J Biol Chem. 2014 Aug 22;289(34):23596-608. doi: 10.1074/jbc.M114.569061. Epub 2014 Jun 26.

Tejera P, et al.
Functional Characterization of Polymorphisms in the Peptidase Inhibitor 3 (Elafin) Gene and Validation of Their Contribution to Risk of Acute Respiratory Distress Syndrome.
Am J Respir Cell Mol Biol. 2014 Aug;51(2):262-72. doi: 10.1165/rcmb.2013-0238OC.

Zheng K, et al.
Influence of glycosylation pattern on the molecular properties of monoclonal antibodies.
MAbs. 2014 May-Jun;6(3):649-58. doi: 10.4161/mabs.28588. Epub 2014 Mar 24.


上海起发实验试剂有限公司
地址:上海浦东川沙镇川沙路6619号上海起发实验试剂有限公司
邮箱:xs1@78bio.com
传真:021-50724961
关注我们
欢迎您关注我们的微信公众号了解更多信息:
欢迎您关注我们的微信公众号
了解更多信息