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progen 61055现货——上海起发

更新时间：2024-03-22 点击次数：151

progen 61055现货——上海起发

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

抗AAV2（完整颗粒）小鼠单克隆抗体

关键功能

纯化、冻干

小鼠单克隆抗体

适用于斑点印迹、ELISA、ICC和中和测定

与AAV2、AAV2 7m8和AAV3完整颗粒反应

同种型：IgG3

Quantity	50 µg
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG3
Conjugate	Unconjugated
Application	Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification	Affinity chromatography
Reactivity	AAV2, AAV2 7m8, AAV3
No reactivity	AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74
Storage before reconstitution	2-8°C until indicated expiry date
Storage after reconstitution	2-8°C
Intended use	Research use only
Clone	A20
Immunogen	AAV2 capsids
Concentration	50 µg/ml after reconstitution with 1 ml dist. water
Formulation	Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Binding affinity	KD value (AAV2) = 2.6E-11 M KD value (AAV3) = <1.0E-12 M
Synonym	Adeno-associated virus 2; AAV-2

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Affinity Chromatography	Assay dependent
Dot Blot	1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA	Assay dependent
Immunoprecipitation (IP)	1:5
Neutralization Assay	EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) - assay dependent

用于表征感染的不同阶段，对分析AAV2组装过程非常有用。A20与AAV2和AAV3、空衣壳和全衣壳特异性反应。识别组装衣壳的构象表位，不存在于变性衣壳蛋白和天然但未组装的衣壳蛋白中。该抗体不能用于免疫印迹。

表位定位实验（Wobus等人，2000）确定了四个免疫反应（不连续）区域。主要反应归因于AAV2衣壳的AA369至aa378序列。该抗体也可用于中和实验。

Wobus，C.E.等。针对腺相关病毒2型（AAV-2）衣壳的单克隆抗体：参与AAV-2细胞相互作用和中和AAV-2感染的衣壳结构域的表位定位和鉴定。J.维罗尔。74, 9281-93 (2000).

Moskalenko，M.等。人类抗腺相关病毒2型中和抗体的表位定位：对Ge的意义