您好!欢迎访问上海起发实验试剂有限公司网站!
全国服务咨询热线:

15921799099

当前位置:首页 > 技术文章 > qiagen Rneasy Mini Kit—RNA提取神器, 快速、高效,轻松搞定高品质RNA

qiagen Rneasy Mini Kit—RNA提取神器, 快速、高效,轻松搞定高品质RNA

更新时间:2025-03-05      点击次数:466

1.jpg

产品名称: 74104 Rneasy Mini Kit (50)

生产商: Qiagen GmbH, Germany

储存与运输: 常温运输,避免高温和阳光直射。

有效期: 见包装标签。


产品简介:

Rneasy Mini Kit 是 Qiagen 公司推出的一款用于从各种生物样本中快速、高效提取高质量总 RNA 的试剂盒。该试剂盒适用于从动物组织、植物组织、细胞、细菌等多种样本中提取 RNA,适用于下游应用如 RT-PCR、qPCR、Northern blotting、cDNA 合成等。


qiagen74104产品组成:

1、RNeasy Mini Spin Columns (50 个)

2、Collection Tubes (2 ml, 50 个)

3、Buffer RLT (裂解缓冲液, 50 ml)

4、Buffer RW1 (洗涤缓冲液, 30 ml)

5、Buffer RPE (洗涤缓冲液, 30 ml)

6、RNase-free Water (无 RNase 水, 10 ml)

7、说明书


储存条件:

*所有组分应储存在室温(15-25°C)下,避免阳光直射。

*Buffer RLT 在使用前应加入 β-巯基乙醇(β-mercaptoethanol),加入后可在室温下储存 1 个月。


qiagen Rneasy Mini Kit操作步骤:

1、样本裂解:

*对于动物组织: 取 30 mg 组织,加入 600 µl Buffer RLT,使用匀浆器匀浆。

*对于细胞: 取 1x10^6 细胞,加入 600 µl Buffer RLT,充分混匀。

*对于植物组织: 取 100 mg 组织,加入 600 µl Buffer RLT,使用匀浆器匀浆。

2、离心:

将裂解后的样本在 4°C 下以 10,000 x g 离心 3 分钟,取上清液。

3、RNA 结合:

将上清液转移到 RNeasy Mini Spin Column 中,以 10,000 x g 离心 30 秒,弃去滤液。

4、洗涤:

*加入 700 µl Buffer RW1 到柱中,以 10,000 x g 离心 30 秒,弃去滤液。

*加入 500 µl Buffer RPE 到柱中,以 10,000 x g 离心 30 秒,弃去滤液。

*重复加入 500 µl Buffer RPE,以 10,000 x g 离心 2 分钟,弃去滤液。

5、洗脱:

将 RNeasy Mini Spin Column 转移到新的 1.5 ml 收集管中,加入 30-50 µl RNase-free Water,以 10,000 x g 离心 1 分钟,收集 RNA。


注意事项:

1、操作过程中应避免 RNase 污染,使用无 RNase 的耗材和试剂。

2、Buffer RLT 含有胍盐,具有刺激性,操作时应佩戴手套和护目镜。

3、提取的 RNA 应尽快用于下游实验或储存于 -80°C。


应用范围:

1、RT-PCR

2、qPCR

3、Northern blotting

4、cDNA 合成


如有任何疑问,欢迎联系上海起发!


注:此中文说明书仅作参考,最终请以说明书为准。



qiagen热卖产品

货号品名规格品牌
74104Rneasy Mini Kit (50)kitqiagen
69506DNeasy Blood & Tissue Kit (250)250qiagen
69504DNeasy Blood and Tissue Kit (50)50 prepsqiagen
12362EndoFree Plasmid Maxi Kit (10)10 QIAGEN-tip 500qiagen
12391EndoFree Plasmid Giga Kit (5)Kitqiagen
51304QIAamp DNA Mini Kit (50)kitqiagen
74106RNeasy Mini Kit (250)eaqiagen
28706试剂盒QIAquick Gel Extraction Kit (250)KITqiagen
76106RNAlater (250ml)250mlqiagen
51306QIAamp DNA Mini Kit (250)250Tqiagen
28704QIAquick Gel Extraction Kit (50)50 reactionsqiagen
27106QIAprep Spin Miniprep Kit (250)250/盒qiagen
73504RNeasy FFPE Kit (50)50次qiagen
19101RNase A (17,500 U)2.5 mlqiagen
52904QIAamp Viral RNA Mini Kit (50)50 Prepsqiagen
79254RNase-Free DNase Set (50)eaqiagen
74136RNeasy Plus Mini Kit (250)250 reactionsqiagen
30210Ni-NTA Agarose (25 ml)25mlqiagen
762174PAXgene Blood RNA Kit (50)50Testqiagen
12183QIAGEN Plasmid Mega Kit (25)25Reactionsqiagen
12381EndoFree Plasmid Mega Kitkitqiagen
57704QIAamp MinElute Virus Spin Kit (50)kitqiagen
56304QIAamp DNA Micro Kit50Testqiagen
28104QIAquick PCR Purification Kit (50)KITqiagen
47014DNeasy PowerSoil Pro Kit (50)50qiagen
51106QIAamp DNA Blood Mini Kit (250)eaqiagen
51804QIAamp PowerFecal Pro DNA KitKitqiagen
74182RNeasy 96 Kit (12)12Testqiagen
51104QIAamp DNA Blood Mini Kit (50)50qiagen
12163QIAGEN Plasmid Maxi Kit (25)25qiagen
12181QIAGEN Plasmid Mega KitKitqiagen
28106QIAquick PCR Purification Kit250rxn/盒qiagen
250001QIAcuity Nanoplate 26k 24-well (10)10Tqiagen
12143QIAGEN Plasmid Midi Kit (25)25qiagen
12191Plasmid Giga KitKitqiagen
129114Nuclease-Free Water (10 x 50 ml)10 x 50 mlqiagen
74134RNeasy Plus Mini Kit (50)50 reactionsqiagen
74804RNeasy Lipid Tissue Mini Kit (50) *组分(1023537+1023539)50Reactionsqiagen
19086Buffer EB250mlqiagen
55114QIAamp Circulating Nucleic Acid KitFor 50 prepsqiagen
12877-50DNeasy PowerClean Cleanup Kit (50)eaqiagen
12888-100DNeasy PowerSoil Kit (100)1Kitqiagen
27104QIAprep Spin Miniprep Kit (50)50次qiagen
12963QIAGEN Plasmid Plus Maxi Kit (25)kitqiagen
74004RNeasy Micro Kit (50)50qiagen
19093Deparaffinization Solution (16ml)16mlqiagen
51185QIAamp DNA Blood Midi Kit (100)15mlqiagen
1031576Buffer TCL (125 ml)125mlqiagen
12162QIAGEN Plasmid Maxi KitKitqiagen
950067QIAcube HT Plasticwarekitqiagen
80204AllPrep DNA/RNA Mini Kit (50)50qiagen
13343Blood & Cell Culture DNA Midi Kit (25)25qiagen
217004miRNeasy Mini KitFor 50 prepsqiagen
12123QIAGEN Plasmid Mini Kit (25)25 kitqiagen
52906QIAamp Viral RNA Mini Kit (250)250 prepqiagen
30230Ni-NTA Agarose (100 ml)100mlqiagen
12866-25RNeasy PowerSoil Total RNA Kit25次/盒qiagen
12945QIAGEN Plasmid Plus Midi Kit (100)eaqiagen
250103QIAcuity Probe PCR Kit (25mL)25Milliliterqiagen
13362Blood & Cell Culture DNA Maxi KitN/Aqiagen
204145QuantiTect SYBR Green PCR Kit(1000x50?l)1000Reactionsqiagen
47016DNeasy PowerSoil Pro Kit (250)250Reactionsqiagen
51404QIAamp Fast DNA Tissue Kit50 prepsqiagen
52304QIAamp RNA Blood Mini Kit (50)EAqiagen
12263QIAfilter Plasmid Maxi Kit (25)25Tqiagen
28506QIAquick PCR & Gel Cleanup Kit (100)100Reactionsqiagen
12888-50DNeasy PowerSoil Kit (50)50 Prepsqiagen
12943QIAGEN Plasmid Plus Kit25 Kitqiagen
59104EpiTect Bisulfite Kit (48)48Reactionsqiagen
250102QIAcuity Probe PCR Kit (5 ml)5mlqiagen
217204miRNeasy Serum/Plasma Advanced Kit (50)50 RNeasyqiagen
217184miRNeasy Serum/Plasma Kit (50)50qiagen
203203HotStarTaq DNA Polymerase (250 U)250uqiagen
20051QIAEX II Gel Extraction Kit (500)500Tqiagen
74034RNeasy Plus Micro Kit (50)50qiagen
19413QIAvac 24 Plusqiagen
74171RNeasy 96 QIAcube HT Kit (5)5Testqiagen
19133QIAGEN Proteinase K (10 ml)1Unitsqiagen
75144RNeasy Midi Kit (50)50Tqiagen
56404QIAamp DNA FFPE Tissue Kit2mlqiagen
28004MinElute PCR Purification Kit2mlqiagen
69989Stainless Steel Beads, 5 mm (200)EAqiagen
206143QIAGEN Multiplex PCR Kit100Tqiagen
763134PAXgene Blood miRNA Kit (50)50Testqiagen
19407VacConnectors (500)500Piecesqiagen
74704RNeasy Fibrous Tissue Mini Kit (50)50Tqiagen
19571AirPore Tape Sheets (50)50Testqiagen
50214QIAamp UCP Pathogen Mini Kit (50)50次qiagen
51604QIAamp Fast DNA Stool Mini Kit (50)50Reactionsqiagen
51704QIAamp DNA Microbiome Kit (50)50次qiagen
28604MinElute Gel Extraction Kit (50)50Reactionsqiagen
26000-50RNeasy PowerMicrobiome Kit (50)KITqiagen
250131QIAcuity OneStep Advanced Probe Kit (1 ml)1mlqiagen
60404QIAamp DSP DNA FFPE Tissue Kit (50)50Reactionsqiagen
210212QIAGEN OneStep RT-PCR Kit (100)100Testqiagen
69997Tungsten Carbide Beads, 3 mm (200)200个/盒qiagen
76104RNAlater (50ml)50mlqiagen
180134GeneRead DNA FFPE Kit (50)50Reactionsqiagen
79216Buffer RLT (220 ml)qiagen
80284AllPrep DNA/RNA Micro Kit (50)eaqiagen





上海起发实验试剂有限公司
地址:上海浦东川沙镇川沙路6619号上海起发实验试剂有限公司
邮箱:xs1@78bio.com
传真:021-50724961
关注我们
欢迎您关注我们的微信公众号了解更多信息:
欢迎您关注我们的微信公众号
了解更多信息