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当前位置:首页 > 技术文章 > USAscientific 1402-4700:八联排管薄壁+无核酸酶,PCR实验新选择

USAscientific 1402-4700:八联排管薄壁+无核酸酶,PCR实验新选择

更新时间:2025-06-24      点击次数:118

USAscientific 1402-4700:八联排管薄壁+无核酸酶,PCR实验新选择

在分子生物学实验中,PCR(聚合酶链式反应)是技术之一,而PCR耗材的稳定性直接影响实验结果的可靠性。今天为大家介绍一款“细节控"实验室的宝藏耗材——USAscientific货号1402-4700,0.2ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八联排无核酸酶薄壁PCR管(以下简称“1402-4700八联排管")。它凭借多项创新设计,成为PCR实验的“效率加速器"。  


产品详细介绍:从结构到材质的“硬核配置"  

1402-4700八联排管是一款专为PCR实验优化的耗材,核心设计围绕“精准、便捷、安全"展开:  

- 品名:0.2 ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八联排无核酸酶薄壁PCR管(0.2ml)

- 结构设计:采用经典8联排条带设计,每条包含8个独立0.2ml PCR管,管体与平盖通过“FLEX-FREE"弹性连接,既保证运输时的稳定性,又支持单手轻松掰取单管,避免传统连盖管“暴力拆条"的麻烦。  

- 材质与工艺:管体采用医疗级聚丙烯(PP)材质,经伽马射线辐照灭菌及无核酸酶处理工艺,确保无DNase/RNase残留(第三方检测报告验证);透明平盖采用相同材质,与管体紧密贴合,耐高温(-20℃~121℃),适配常规PCR热循环仪。  

- 参数规格:单管容量0.2ml(±0.5%误差),管壁厚度仅0.15mm(薄壁设计),外径12mm,高度47mm,符合主流PCR仪(如ABI 7500、Bio-Rad CFX系列等)的孔板适配要求。  


核心优势:为什么它是PCR实验的“优选"?  

1. 无核酸酶污染风险:通过严格的去核酸酶工艺(辐照+化学处理),消除管壁残留的核酸酶,降低实验假阳性概率,尤其适用于高灵敏度实验(如qPCR、痕量DNA扩增)。  

2. 薄壁设计,提升扩增效率:0.15mm超薄管壁导热更快,减少热循环仪升降温时间(实测可缩短10%-15%扩增时间),同时减少温度梯度差异,提高扩增一致性。  

3. FLEX-FREE结构,操作更便捷:连排管体通过弹性连接固定,既避免运输脱落,又能单手掰取单管,无需剪刀或暴力拆分,减少耗材浪费,尤其适合高通量实验场景。  

4. 透明平盖,观察与密封兼顾:平盖材质高透光,可直接观察管内反应液体积及颜色变化;同时平盖与管体紧密贴合,经压力测试(500g压力)无液体泄漏,有效防止气溶胶污染,适配自动化移液设备。  

5. 全场景适配,兼容性强:无论是常规PCR、qPCR、逆转录PCR,还是基因测序、克隆验证,1402-4700八联排管均表现稳定,是实验室日常PCR实验的“万能搭档"。  


常见疑问与解答  

Q1:产品真的“无核酸酶"吗?如何验证?  

A:是的。1402-4700八联排管采用伽马射线辐照灭菌联合无核酸酶处理工艺,出厂前经第三方检测(如Taq酶消化实验),确认无DNase/RNase残留,可提供完整质检报告。  

Q2:薄壁设计会不会容易破裂?  

A:不会。管体采用高韧性医疗级PP材质,虽壁厚仅0.15mm,但抗冲击性强(经-20℃低温脆化测试无破裂),可承受121℃高压灭菌(15分钟)及反复热循环,耐用性优于普通厚壁管。  

Q3:透明平盖的密封性如何?会不会漏液?  

A:平盖与管体通过模压工艺紧密结合,经压力测试(500g静置30分钟)无液体渗漏,且表面光滑无凹陷,可有效防止气溶胶污染,适配移液枪、自动化工作站等设备。  

Q4:是否适用于所有品牌PCR仪?  

A:是的。管体尺寸严格符合国际标准(直径12mm,高度47mm),兼容主流品牌热循环仪(如ABI、Bio-Rad、Eppendorf、Roche等),无需额外调整孔板适配器。  

Q5:可以高温灭菌吗?灭菌后性能会变化吗?  

A:支持121℃高压灭菌(15分钟),灭菌后管体无变形、无材质老化,无核酸酶活性残留,不影响后续实验性能。  

Q6:与普通八联排管相比,优势在哪里?  

A:普通八联排管可能存在核酸酶污染风险(未做去酶处理),且多为厚壁设计(影响扩增效率);此外,普通连盖管拆条时易断裂,浪费耗材。1402-4700八联排管通过无酶处理、薄壁设计及FLEX-FREE结构,综合解决了这些问题。  

Q7:包装规格是多少?  

A:每盒含100条八联排管(即800个单管),每条独立塑封包装,方便取用和存储,避免交叉污染。  

Q8:存储条件有什么要求?  

A:未开封时建议常温(15-30℃)干燥保存,避免阳光直射;开封后需密封保存,建议6个月内用完,以保证最佳性能。  

Q9:保质期多久?  

A:未开封状态下保质期为2年(自生产日期起算),产品包装标注具体生产日期及有效期。  

Q10:是否兼容qPCR染料(如SYBR Green)?  

A:是的。管体材质高透明、无荧光淬灭,可清晰观察qPCR反应体系的荧光信号,适用于SYBR Green、TaqMan等主流荧光染料。  


(注:本文内容基于品牌公开资料及行业常规信息整理,具体以品牌信息文档为准。)


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