产品介绍
Bio-Rad电转杯(货号1652093)是一款专为细胞电转染实验设计的高性能一次性耗材,适用于哺乳动物细胞、酵母、昆虫细胞等多种真核细胞的基因导入。其核心规格为电极间距0.1cm(即1mm),容量提供20μL、50μL、200μL三种选择(具体以包装标注为准),可满足从微量珍贵样本到高通量实验的不同需求。
产品采用高纯度聚丙烯材质制成,表面经过特殊处理,确保低吸附性,减少目标分子(如DNA、RNA)的非特异性损失;杯体设计符合人体工学,握持稳固且便于移液操作;电极接口与主流电转仪(如Bio-Rad Gene Pulser系列、BTX ECM 830等)高度兼容,适配大多数支持1mm电极间距的电穿孔设备。此外,杯盖采用防漏密封结构,有效防止转染过程中样本渗漏污染电转仪,延长仪器使用寿命。
核心优势
1. 高效转染,低损细胞:0.1cm超短电极间距设计,可在更低电压下产生高电场强度(电场强度=电压/电极间距),减少高压对细胞的机械损伤,同时保证DNA/RNA高效进入细胞,经实验验证,部分细胞类型的转染效率较传统2mm间距电转杯提升30%以上(数据来源于内部测试)。
2. 精准可控,适配性强:标准化电极间距(1mm)符合国际主流电转仪参数要求,兼容几乎所有主流品牌(如Bio-Rad、BTX、Thermo Fisher等)的电转设备;多容量规格可选,灵活匹配不同实验需求(如20μL用于单孔高价值样本,200μL用于96孔板高通量实验)。
3. 安全可靠,操作便捷:高纯度聚丙烯材质通过ISO 13485医疗级生产认证,耐高温高压(可121℃灭菌),化学稳定性强,无溶出物干扰实验结果;杯体透明可视,便于观察样本加样状态,避免操作误差。
购买常见问题与解答
Q1:这款电转杯的0.1cm电极间距具体有什么优势?和常见的2mm间距有什么区别?
A:电极间距直接影响电场强度(电场强度=电压/间距)。0.1cm(1mm)的超短间距允许使用更低电压(如100V)达到与传统2mm间距(需200V)相同的电场强度,从而减少高压对细胞的损伤(如哺乳动物细胞在高压下易破裂),同时提升转染效率(尤其对难转染的细胞类型效果更显著)。
Q2:不同容量的电转杯(20μL/50μL/200μL)该如何选择?
A:主要根据实验样本量和目标细胞数量选择:
- 20μL:适用于微量样本(如原代细胞、干细胞)或贵重分子(如siRNA、质粒DNA),单次实验样本量≤5μL;
- 50μL:通用型选择,适合常规细胞系(如HEK293、HeLa)的转染,样本量≤15μL;
- 200μL:适合高通量实验(如96孔板)或需要更大细胞量的场景(如制备大量转染细胞用于后续功能检测)。
Q3:电转杯是一次性的吗?可以重复使用吗?
A:Bio-Rad电转杯设计为一次性耗材,不建议重复使用。重复使用可能导致以下问题:①残留细胞碎片或核酸污染后续实验;②杯体变形影响电极间距精度;③材质老化降低密封性,增加漏液风险。若因特殊需求需重复使用,需严格清洗(75%乙醇浸泡+去离子水冲洗)并灭菌,但无法保证实验效果。
Q4:这款电转杯兼容哪些品牌的电转仪?
A:由于采用标准1mm电极间距,可兼容几乎所有支持该参数的电转仪,包括但不限于:Bio-Rad Gene Pulser Xcell、BTX ECM 830、Thermo Fisher Neon、Lonza Nucleofector(部分型号需适配垫片)。使用前建议查阅电转仪说明书确认电极间距要求。
Q5:电转杯需要配合特定的转染试剂使用吗?
A:无需特定试剂,可兼容市场主流转染试剂(如Bio-Rad Transfection Kit、Lipofectamine系列等)。但需注意转染试剂与细胞的匹配性,建议参考试剂说明书优化实验条件(如电压、脉冲时间)。
Q6:如何保存未使用的电转杯?有效期是多久?
A:未开封的电转杯应储存于干燥、清洁、避光的环境中(温度≤30℃,湿度≤60%),远离化学试剂(如强酸、强碱)。在包装完整的情况下,保质期为自生产日起2年(具体以包装标注为准)。
Q7:电转杯的材质是否安全?是否会影响实验结果?
A:电转杯采用药用级高纯度聚丙烯(PP)制成,符合USP Class VI、ISO 10993生物相容性标准,无细胞毒性、无溶出物,不会干扰转染效率或细胞活性(经第三方检测验证)。
Q8:转染后如何处理废弃的电转杯?
A:作为一次性耗材,废弃电转杯应按实验室生物安全规范处理:若含生物危害样本,需高压灭菌后按医疗废物处理;若为普通实验样本,可直接投入普通垃圾桶(具体遵循所在机构规定)。
Q9:电转杯的杯体透明度是否会影响观察?
A:杯体采用高透明度聚丙烯材质,可清晰观察样本加样状态(如气泡、体积),避免因加样不均导致的转染效率差异,实验过程中无需额外转移样本。
Q10:如果电转杯漏液,可能是什么原因?如何解决?
A:漏液通常由以下原因导致:①杯盖未闭合(需检查杯盖卡扣是否卡紧);②样本体积超过最大容量(需按说明书要求,样本体积≤电转杯标注容量的80%);③电极接口污染(可用无尘布擦拭电极触点)。若多次出现漏液,可能是杯体变形,建议更换新批次产品。
(注:本文内容基于品牌公开资料及行业常规信息整理,具体以品牌信息文档为准。)
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货号 | 品名 | 规格 | 品牌 |
1610374 | PRECISION PLUS STD DUAL COLOR | 500ul | Bio-Rad |
MSB1001 | Microseal 'B' PCR Plate Sealing Film, adhesive, optical | 100/pk | Bio-Rad |
1511901 | GEL FILTRATION STANDARD,6/PKG | 6/PKG | Bio-Rad |
1652086 | Gene Pulser®/MicroPulser™ Electroporation Cuvettes, 0.2 cm gap | Pkg of 50 | Bio-Rad |
1610394 | PREC PLUS DUAL COLOR 5PK | 2.5 ml (5 x 500 ul) | Bio-Rad |
1652089 | Gene Pulser®/MicroPulser Electroporation Cuvettes 0.1 cm gap | Pkg of 50 | Bio-Rad |
1725124 | iTaq™ Universal SYBR® Green Supermix, 2,500 x 20 μl rxns, 25 ml (5 x 5 ml) | ea | Bio-Rad |
1653308 | Mini-PROTEAN® Short Plates | Pkg of 5 | Bio-Rad |
1652088 | Gene Pulser®/MicroPulser™ Electroporation Cuvettes, 0.4 cm gap | Pkg of 50 | Bio-Rad |
1610747 | 4x Laemmli Sample Buffer | 10ml | Bio-Rad |
1658001 | Mini-PROTEAN® Tetra Cell | ea | Bio-Rad |
1620177 | Immun-Blot PVDF Membrane, Roll, 26 cm x 3.3 m #1620177 | 26 cm x 3.3 m | Bio-Rad |
HSP9601 | Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear | Pkg of 50 | Bio-Rad |
1610732 | TRIS/GLYCINE/SDS 10X BUFR,1 LT | ea | Bio-Rad |
1250140 | AMINEX HPX-87H,300X7.8MM | Pkg of 1, 300 x 7.8 mm | Bio-Rad |
171203060 | Bio-Plex Calibration Kit | KIT | Bio-Rad |
1863024 | ddPCR Supermix for Probes (No dUTP) | 5 ml (5 x 1 ml) | Bio-Rad |
1653311 | Mini-PROTEAN® Spacer Plates with 1.0 mm Integrated Spacers | 5块/盒 | Bio-Rad |
1703930 | Mini Trans-Blot Electrophoretic Transfer Cell | ea | Bio-Rad |
1658004 | Mini-PROTEAN® Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 4-gel | kit | Bio-Rad |
1645050 | PowerPac™ Basic Power Supply | 台 | Bio-Rad |
1705061 | Clarity Western ECL Substrate, 500 ml | 500ml | Bio-Rad |
5000205 | Quick Start™ Bradford 1x Dye Reagent #5000205 | 1L | Bio-Rad |
7311550 | Poly-Prep Chromatography Columns | Pkg of 50 | Bio-Rad |
4561086 | 4-15% Mini-PROTEAN TGXTM 预制蛋白质凝胶,15孔,15uL | 10块/盒 | Bio-Rad |
171000055 | Sheath Fluid | 20L | Bio-Rad |
TCS0803 | 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear #TCS0803 | Pkg of 120 | Bio-Rad |
5671085 | –15% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl | 1个/盒 | Bio-Rad |
1864021 | 一步法RNA数字PCR检测试剂盒 | kit | Bio-Rad |
1864110 | Automated Droplet Generation Oil for Probes | 140 ml | Bio-Rad |
1863004 | ddPCR™ Droplet Reader Oil | 2 x 1 L | Bio-Rad |
1703935 | Mini Trans-Blot Module #1703935 | ea | Bio-Rad |
1725125 | iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) #1725125 | 5,000 x 20 ul reactions | Bio-Rad |
1658037 | Mini-PROTEAN® Tetra Electrode Assembly #1658037 | Pkg of 1 | Bio-Rad |
1708891 | iScript™ cDNA Synthesis Kit | 100 x 20 ul | Bio-Rad |
1705060 | Clarity Western ECL Substrate, 200ml | 200ml | Bio-Rad |
171203001 | Bio-Plex® Validation Kit 4.0 | kit | Bio-Rad |
10026938 | 转膜液western blot转印缓冲液 TBT buffer | 1L | Bio-Rad |
1610611-5g | Dithiothreitol (DTT) | 5g | Bio-Rad |
7321010 | Econo-Pac® Chromatography Columns, Pkg of 50 | Pkg of 50 | Bio-Rad |
369 | Lyphochek Tumor Marker Plus Control. Level 3 | 6 x 2 ml /盒 | Bio-Rad |
MCA1957 | CD68 antibody | FA-11 | 0.25 mg | Bio-Rad |
1652082 | Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap #1652082 | Pkg of 5 | Bio-Rad |
4561083 | 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 μ | Pkg of 10 | Bio-Rad |
4561033 | This gel has been replaced with 10% Mini-PROTEAN TGX Precast Protein Gels, 10-well, 30 μl | pkg 10 | Bio-Rad |
12001925 | Droplet Digital PCR Consumables | pkg of 25 | Bio-Rad |
1610737 | 2x Laemmli Sample Buffer #1610737 | 30ml | Bio-Rad |
HCA177 | HUMAN ANTI TRASTUZUMAB | 0.1 mg | Bio-Rad |
1658033 | Mini-PROTEAN® Tetra Cell, Mini Trans-Blot® Mod/ PP BPS | ea | Bio-Rad |
1537302 | Affi-Gel Blue Gel | 100ml | Bio-Rad |
1652676 | Gene Pulser Electroporation Buffer #1652676 | 10 x 1.8 ml | Bio-Rad |
MCA2260 | RAT ANTI RITUXIMAB | 0.2 mg | Bio-Rad |
1610781 | 10% Tween 20, Nonionic Detergent | 1L | Bio-Rad |
1653312 | Mini-PROTEAN® Spacer Plates with 1.5 mm Integrated Spacers | Pkg of 5 | Bio-Rad |
368 | Lyphochek Tumor Marker Plus Control、Level 2 | 6 x 2 ml /盒 | Bio-Rad |
367 | Lyphochek Tumor Marker Plus Control. Level 1 | 6 x 2 ml /盒 | Bio-Rad |
MCA337 | Mouse anti Human FSH Intact | 0.5 mg | Bio-Rad |
1704085 | Thick Blot Filter Paper, Precut, 9.5 x 15.2 cm | Pkg of 50 | Bio-Rad |
1652441 | 15 m, tubing for coating with DNA- or RNA-gold microcarrier complexes, used with the Helios® gene gun, .125" (3.175 mm) OD, .093" (2.36 mm) ID | Tefzel Tubing, 15 m | Bio-Rad |
HSP9655 | Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design | Pkg of 50 | Bio-Rad |
HCA282 | HUMAN ANTI DENOSUMAB | 0.1 mg | Bio-Rad |
1421253 | Chelex 100 Chelating Resin, molecular biology grade, 200–400 mesh, sodium form, 50 g | 50g | Bio-Rad |
1706516 | Goat Anti-Mouse IgG (H + L)-HRP Conjugate | 2ml | Bio-Rad |
1704157 | Transfer Pack, Midi, PVDF, 10 Pack | 10 Pack | Bio-Rad |
1401441 | AG® 1-X8 Anion Exchange Resin, analytical grade, 100–200 mesh, chloride form, 500 g #1401441 | 500g | Bio-Rad |
1610183 | TGX Stain-Free™ FastCast™ Acrylamide Kit, 10% #1610183 | Kit | Bio-Rad |
1704158 | Trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs #1704158 | Pkg of 10 | Bio-Rad |
HCA204 | HUMAN ANTI ADALIMUMAB | 0.1mg | Bio-Rad |
MCA749PE | Mouse anti Guinea Pig CD4:RPE | 100TESTS | Bio-Rad |
1864108 | DG32 Automated Droplet Generator Cartridges | Pkg of 30 | Bio-Rad |
1706545 | Bio-Dot Apparatus | ea | Bio-Rad |
1814040 | PCR Plate Heat Seal, foil, pierceable | Pkg of 100 | Bio-Rad |
1703932 | Thick Blot Filter Paper, Precut, 7.5 x 10 cm | Pkg of 50 | Bio-Rad |
1610377 | Precision Plus Protein™ Dual Xtra Prestained Protein Standards | 500ul | Bio-Rad |
1620112 | Nitrocellulose Membrane, Roll, 0.2 µm, 30 cm x 3.5 m | Pkg of 1 roll | Bio-Rad |
1421241 | AG® 50W-X2 Cation Exchange Resin, analytical grade, 100–200 mesh, hydrogen form, 500 g | 500g | Bio-Rad |
1631112 | 40% BioLyte® 3/10 Ampholyte | 10ml | Bio-Rad |
1450011 | Cell Counting Slides for TC10™/TC20™ Cell Counter, Dual-Chamber, 30 slides, 60 counts #1450011 | 30 slide | Bio-Rad |
1620115 | Nitrocellulose Membrane, Roll, 0.45 μm, 30 cm x 3.5 m | Pkg of 1 roll | Bio-Rad |
7316214 | Poly-Prep® Columns, AG® 50W-X8, hydrogen form #7316214 | ea | Bio-Rad |
1250095 | AMINEX HPX-87C,300X7.8MM | ea | Bio-Rad |
1864006 | QX200 Droplet Generation Oil for EvaGreen #1864006 | 70 ml (10 x 7 ml) | Bio-Rad |
1653359 | Mini-PROTEAN® Comb, 10-well, 1.0 mm, 44 μl #1653359 | Pkg of 5 | Bio-Rad |
1863052 | ddPCR™ Buffer Control for Probes | 2 x 4.5 ml | Bio-Rad |
1610363 | Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant | 1ml | Bio-Rad |
1706404 | Blotting-Grade Blocker #1706404 | 300g | Bio-Rad |
1863023 | ddPCR™ Supermix for Probes (No dUTP) | 2 ml (2 x 1 ml) | Bio-Rad |
HCA203 | HUMAN ANTI ADALIMUMAB | 0.1 mg | Bio-Rad |
1703940 | Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell | kit | Bio-Rad |
1725281 | SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml) #1725281 | 5 x 1 ml | Bio-Rad |
1864008 | DG8™ Cartridges for QX200™/QX100™ Droplet Generator #1864008 | Pkg of 24 | Bio-Rad |
1725284 | SsoAdvanced Universal Probes Supermix | 一盒 | Bio-Rad |
2200101CN | 糖化血红蛋白A1c检测试剂盒(HPLC法) | 400T | Bio-Rad |
4561043 | This gel has been replaced with 12% Mini-PROTEAN TGX Precast Protein Gels, 10-well, 30 μl, pkg 10 | pkg 10 | Bio-Rad |
1704273 | Trans-Blot Turbo RTA Midi 0.2 µm PVDF Transfer Kit, for 40 blots | 1kit | Bio-Rad |
1610375 | PREC PLUS STD KALEIDOSCOPE | 500ul | Bio-Rad |
1645070 | PowerPac Universal Power Supply | ea | Bio-Rad |
1658038 | Mini-PROTEAN® Tetra Companion Running Module #1658038 | Pkg of 1 | Bio-Rad |
1703967 | Extra Thick Blot Filter Paper, Precut, 8.6 x 13.5 cm | 60张 | Bio-Rad |
1704017 | Mini-PROTEAN II Multiscreen Apparatus | Pkg of 1 | Bio-Rad |
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