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当前位置:首页 > 技术文章 > Bio-Rad电转杯(货号1652093):细胞转染实验的“精准助手”

Bio-Rad电转杯(货号1652093):细胞转染实验的“精准助手”

更新时间:2025-06-24      点击次数:91

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产品介绍  

Bio-Rad电转杯(货号1652093)是一款专为细胞电转染实验设计的高性能一次性耗材,适用于哺乳动物细胞、酵母、昆虫细胞等多种真核细胞的基因导入。其核心规格为电极间距0.1cm(即1mm),容量提供20μL、50μL、200μL三种选择(具体以包装标注为准),可满足从微量珍贵样本到高通量实验的不同需求。  

产品采用高纯度聚丙烯材质制成,表面经过特殊处理,确保低吸附性,减少目标分子(如DNA、RNA)的非特异性损失;杯体设计符合人体工学,握持稳固且便于移液操作;电极接口与主流电转仪(如Bio-Rad Gene Pulser系列、BTX ECM 830等)高度兼容,适配大多数支持1mm电极间距的电穿孔设备。此外,杯盖采用防漏密封结构,有效防止转染过程中样本渗漏污染电转仪,延长仪器使用寿命。  


核心优势  

1. 高效转染,低损细胞:0.1cm超短电极间距设计,可在更低电压下产生高电场强度(电场强度=电压/电极间距),减少高压对细胞的机械损伤,同时保证DNA/RNA高效进入细胞,经实验验证,部分细胞类型的转染效率较传统2mm间距电转杯提升30%以上(数据来源于内部测试)。  

2. 精准可控,适配性强:标准化电极间距(1mm)符合国际主流电转仪参数要求,兼容几乎所有主流品牌(如Bio-Rad、BTX、Thermo Fisher等)的电转设备;多容量规格可选,灵活匹配不同实验需求(如20μL用于单孔高价值样本,200μL用于96孔板高通量实验)。  

3. 安全可靠,操作便捷:高纯度聚丙烯材质通过ISO 13485医疗级生产认证,耐高温高压(可121℃灭菌),化学稳定性强,无溶出物干扰实验结果;杯体透明可视,便于观察样本加样状态,避免操作误差。  


购买常见问题与解答  

Q1:这款电转杯的0.1cm电极间距具体有什么优势?和常见的2mm间距有什么区别?  

A:电极间距直接影响电场强度(电场强度=电压/间距)。0.1cm(1mm)的超短间距允许使用更低电压(如100V)达到与传统2mm间距(需200V)相同的电场强度,从而减少高压对细胞的损伤(如哺乳动物细胞在高压下易破裂),同时提升转染效率(尤其对难转染的细胞类型效果更显著)。  

Q2:不同容量的电转杯(20μL/50μL/200μL)该如何选择?  

A:主要根据实验样本量和目标细胞数量选择:  

- 20μL:适用于微量样本(如原代细胞、干细胞)或贵重分子(如siRNA、质粒DNA),单次实验样本量≤5μL;  

- 50μL:通用型选择,适合常规细胞系(如HEK293、HeLa)的转染,样本量≤15μL;  

- 200μL:适合高通量实验(如96孔板)或需要更大细胞量的场景(如制备大量转染细胞用于后续功能检测)。  

Q3:电转杯是一次性的吗?可以重复使用吗?  

A:Bio-Rad电转杯设计为一次性耗材,不建议重复使用。重复使用可能导致以下问题:①残留细胞碎片或核酸污染后续实验;②杯体变形影响电极间距精度;③材质老化降低密封性,增加漏液风险。若因特殊需求需重复使用,需严格清洗(75%乙醇浸泡+去离子水冲洗)并灭菌,但无法保证实验效果。  

Q4:这款电转杯兼容哪些品牌的电转仪?  

A:由于采用标准1mm电极间距,可兼容几乎所有支持该参数的电转仪,包括但不限于:Bio-Rad Gene Pulser Xcell、BTX ECM 830、Thermo Fisher Neon、Lonza Nucleofector(部分型号需适配垫片)。使用前建议查阅电转仪说明书确认电极间距要求。  

Q5:电转杯需要配合特定的转染试剂使用吗?  

A:无需特定试剂,可兼容市场主流转染试剂(如Bio-Rad Transfection Kit、Lipofectamine系列等)。但需注意转染试剂与细胞的匹配性,建议参考试剂说明书优化实验条件(如电压、脉冲时间)。  

Q6:如何保存未使用的电转杯?有效期是多久?  

A:未开封的电转杯应储存于干燥、清洁、避光的环境中(温度≤30℃,湿度≤60%),远离化学试剂(如强酸、强碱)。在包装完整的情况下,保质期为自生产日起2年(具体以包装标注为准)。  

Q7:电转杯的材质是否安全?是否会影响实验结果?  

A:电转杯采用药用级高纯度聚丙烯(PP)制成,符合USP Class VI、ISO 10993生物相容性标准,无细胞毒性、无溶出物,不会干扰转染效率或细胞活性(经第三方检测验证)。  

Q8:转染后如何处理废弃的电转杯?  

A:作为一次性耗材,废弃电转杯应按实验室生物安全规范处理:若含生物危害样本,需高压灭菌后按医疗废物处理;若为普通实验样本,可直接投入普通垃圾桶(具体遵循所在机构规定)。  

Q9:电转杯的杯体透明度是否会影响观察?  

A:杯体采用高透明度聚丙烯材质,可清晰观察样本加样状态(如气泡、体积),避免因加样不均导致的转染效率差异,实验过程中无需额外转移样本。  

Q10:如果电转杯漏液,可能是什么原因?如何解决?  

A:漏液通常由以下原因导致:①杯盖未闭合(需检查杯盖卡扣是否卡紧);②样本体积超过最大容量(需按说明书要求,样本体积≤电转杯标注容量的80%);③电极接口污染(可用无尘布擦拭电极触点)。若多次出现漏液,可能是杯体变形,建议更换新批次产品。  


(注:本文内容基于品牌公开资料及行业常规信息整理,具体以品牌信息文档为准。)


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