Hypoxyprobe™ Omni Kit说明书

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Hypoxyprobe™ Omni Kit

HPI Catalog # HP3-XXX

Kit contents: Solid pimonidazole HCl (Hypoxyprobe™-1) and affinity purified rabbit anti-                               pimonidazole antibody (PAb2627AP)(ca 0.5 mg/mL IgG)

Applications: Immunochemical detection of cell and tissue hypoxia through immunofluorescence                      or immunoperoxidase staining, western blotting, or flow cytometry. Very low                               background in mouse tissues when the Hypoxyprobe™-1 Omni Kit is combined                         with an anti-rabbit secondary reagent (secondary reagent not supplied in the                               Omni Kit).

Quantities:     a.  Hypoxyprobe™-1 Omni Kits contain100 mg, 200 mg or 1000 mg of                                    Hypoxyprobe™- 1 (pimonidazole HCl). Typical doses are 60mg/kg body weight                        for small animal studies and 14mg/kg body weight for human studies

        b. One vial (100 and 200 mg Kits) or two vials (1000 mg Kit) of 200 microliters                         of affinity purified anti-pimonidazole rabbit antisera containing 0.09% sodium                               azide and 1% BSA as stabilizers. Optimal dilution of PAb2627AP is to be                                  determined by each investigator but a 1/50 -1/200 dilution has been found to give                       strong immunostaining in mouse tumor tissue when combined with peroxidase                              conjugated goat anti-rabbit antisera.

Not supplied: Secondary anti-rabbit reagent and standard IHC reagents such as buffers,                                  substrate chromogens, etc.

Storage: a. Store Hypoxyprobe™-1 solid at room temperature in the dark (can also be stored                  at 2- 8 degrees C).

              b. Store rabbit PAb2627AP at 2-8 degrees C. Do not freeze

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