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Activator | Source | Application | Remarks |
|
| ||
113-01 / 113-04 | Protac® | • Determination of protein C and protein S | Serine protease Agkistrodon contortrix |
116-01 | Ecarin | • Prothrombin assay | Metalloprotease, activates specifically prothrombin via meizothrombin, does not need PL and Ca2+ Echis carinatus |
119-02 | Convulxin | Activation of human plaets via binding and clustering of GPVI-receptors under physiological conditions | Crotalus durissus |
121-03 | RVV Factor V-Activator | Activation of FV in APC-R assay | Serine protease, not inhibited by AT |
121-06 | RVV-X | • Determination of FX | Metalloprotease, does also (weakly) activate protein C and factor IX Daboia (Vipera) russelli |
800191 | Pefakit® Reptilase® Time | Ready-to-use diagnostic reagent for measurement of | CE-marked IVD Bothrops atrox |
中文版：
活化剂 | 资源 | 应用 | 备注 |
113-01 / 113-04 | Protac® | •蛋白C和蛋白S的 | 丝氨酸蛋白酶Agkistrodon contortrix |
116-01 | 蝰蛇毒 | •凝血酶原测定 | 金属蛋白酶，通过凝血酶原特异性激活凝血酶原，不需要PL和Ca2 + Echis carinatus |
119-02 | 惊厥 | 在生理条件下通过结合和聚集GPVI-受体激活人血小板 | 响尾蛇durissus |
121-03 | RVV因子V激动剂 | 在APC-R测定中激活FV | 丝氨酸蛋白酶，不受AT抑制 |
121-06 | RVV-X | •FX的测定 | 金属蛋白酶，也（弱）激活蛋白C和因子IX Daboia（Vipera）russelli |
800191 | Pefakit®Reptilase®时间 | 即用型诊断试剂，用于测量 | CE标记的IVD Bothrops atrox |
附件为113-01说明书。
Description: Protac, a single chain glycoprotein, is a fast-acting protein C activator isolated from the venom of the copperhead snake Agkistrodon contortrix and closely related snake species. This serine proteinase rapidly converts protein C of man and other vertebrates into activated protein C which may be determined either by measuring its prolonging effect on the activated partial thromboplastin time (APTT) or by measuring its enzyme activity by means of a specific
| chromogenic substrate. |
|
Application: |
Determination of protein C and protein S levels. | |
Origin: |
Agkistrodon contortrix snake venom |
MW: 36’000 to 42’000 |
Assay: |
Chromogenic method |
Clotting method |
| 0.050 ml human citrated plasma | 0.025 ml human citrated plasma |
| 0.050 ml Protac (0.5 unit/ml) | 0.025 ml Protac (0.1 unit/ml) |
| => incubate for 5 min at 37°C | 0.025 ml Actin |
| 0.800 ml 50 mM Tris-HCl buffer, pH 8.4 | => incubate for 4 min at 37°C |
| 0.100 ml PefachromePCa (4 mM) => Determination of DOD/min at 405 nm | 0.025 ml 25 mM CaCl2 => Determination of clotting time |
Unit definition: 1 Unit is the amount of Protac which activates the amount of protein C contained in 1 ml of normal citrated human plasma within 5 min at 37°C.
Stability: | Lyophilised vials: Reconstituted vials: | 5 years 30 days at 2 – 8°C, approx. 7 days at 37°C |
|
| 1 year at -20°C, 1 month at 20°C. |
Storage: May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2 - 8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.
References: Bastian S. Protein C-Diagnostik – Methoden, Interpretation und Limitationen. ellipse 2002; 18 (1): 11-16
Gempeler-Messina PM, Volz K, Bühler B, Müller C. Protein C Activators from Snake Venoms and Their Diagnostic Use. Haemostasis 2001; 31:266-72.
Robert A, Eschwege V, Hameg H, Drouet L, Aillaud MF. Anticoagulant response to Agkistrodon contortrix venom (ACV test): a new global test to screen for defects in the anticoagulant protein C pathway. Thromb Haemost 1996; 75: 562-66.
Kraus M, Noah M, Fickenscher K. The PCAT - a simple screening assay for assessing the functionality of the protein C anticoagulant pathway. Thromb Res 1995; 79: 217-22.
Meier J, Stocker K. Snake venom protein C activators. In: Handbook of Natural Toxins, Reptile Venoms and Toxins. Tu AT, ed. New York: Marcel Dekker 1990; 265-79.
Stocker K, Fischer H, Meier J, Brogli M, Svendsen L. Characterization of the protein C activator Protac from the venom of the southern copperhead (Agkistrodon contortrix) snake. Toxicon 1987; 25: 239-52.
Package size: | Vial containing 3 unit/vial Code: | 113-01 |
| Bulk [unit] | 113-04 |
| FOR RESEARCH USE ONLY. NOT FOR HUMAN USE OR DRUG USE. |
|
Pentapharm 2018年产品列表
货号 | 品名 | 规格 | 目录价 | 品牌 |
081-01 | Pefachrome® TH 5244 | 25 mg | 3000.00 | Pentapharm |
081-05 | Pefachrome® TH 5247 | 25 mg | 3000.00 | Pentapharm |
081-09 | Pefachrome® TH 5251 | 25 mg | 3000.00 | Pentapharm |
081-11 | Pefachrome® FXIIa/TH 5253 | 25 mg | 3000.00 | Pentapharm |
081-45 | Pefachrome® 6017 | 10 µmol | 3000.00 | Pentapharm |
081-15 | Pefachrome® TH 5256 | 25 mg | 3000.00 | Pentapharm |
081-17 | Pefachrome® TG | 25 mg | 3000.00 | Pentapharm |
081-66 | Pefachrome® TH 8198 | 25 mg | 3000.00 | Pentapharm |
082-33 | Pefachrome® uPA 8294 | 25 mg | 3000.00 | Pentapharm |
083-01 | Pefachrome® PL/Tryp 5261 | 25 mg | 3000.00 | Pentapharm |
083-02 | Pefachrome® PL 5262 | 25 mg | 3000.00 | Pentapharm |
083-03 | Pefachrome® PL 5263 | 25 mg | 3000.00 | Pentapharm |
083-04 | Pefachrome® PL 5264 | 25 mg | 3000.00 | Pentapharm |
084-01 | Pefachrome® TRY 5274 | 25 mg | 3000.00 | Pentapharm |
085-01 | Pefachrome® FXa 5277 | 25 mg | 3000.00 | Pentapharm |
085-03 | Pefachrome® FXa 5279 | 25 mg | 3000.00 | Pentapharm |
085-06 | Pefachrome® FXa/LAL 5288 | 25 mg | 3000.00 | Pentapharm |
086-11 | Pefachrome® LAL 5288 | 100 μmol | 6000.00 | Pentapharm |
085-27 | Pefachrome® FXa 8595 | 25 mg | 3000.00 | Pentapharm |
802050 | Pefachrome® FXa 2732 | 25 mg | 3000.00 | Pentapharm |
087-03 | Pefachrome® C1E | 25 mg | 3000.00 | Pentapharm |
089-02 | Pefachrome® PCa | 25 mg | 3000.00 | Pentapharm |
091-01 | Pefachrome® tPA | 25 mg | 3000.00 | Pentapharm |
091-03 | Pefachrome® tPA5312 | 25 mg | 3000.00 | Pentapharm |
093-01 | Pefachrome® FVIIa | 25 mg | 3000.00 | Pentapharm |
095-20 | Pefachrome® FIXa | 10 μmol | 1500.00 | Pentapharm |
801058 | Pefafluor® TH | 25 mg | 3600.00 | Pentapharm |
073-70 | Aprotinin Conc. Solution (vial 50 ml) | Mio KIU | 1500.00 | Pentapharm |
800277 | Aprotinin Powder, lyoph. (vial 1 g) | Mio KIU | 1200.00 | Pentapharm |
126-10 | r-Hirudin EC | 2000 ATU | 3200.00 | Pentapharm |
381-01 | Pefabloc® TH (α-NAPAP) | 5 mg | 1800.00 | Pentapharm |
399-01 | Pefabloc® SC | 1 g | 2200.00 | Pentapharm |
399-01 | Pefabloc® SC | 10 g | 20000.00 | Pentapharm |
099-01 | Pefabloc® FG | 1 g | 11600.00 | Pentapharm |
099-11 | Pefabloc® FG | 50 mg | 1200.00 | Pentapharm |
101-04 | Batroxobin maranhao | 100 BU | 2500.00 | Pentapharm |
101-06 | Batroxobin maranhao | 1000 BU | 22500.00 | Pentapharm |
113-01 | Protac® | 3 U | 780.00 | Pentapharm |
113-05 | Protac® solution 10 U/ml | 1 ml | 2400.00 | Pentapharm |
116-01 | Ecarin | 50 EU | 1980.00 | Pentapharm |
119-02 | Convulxin | 50 µg | 8600.00 | Pentapharm |
121-03 | RVV-Factor V Activator | 1000 U | 6000.00 | Pentapharm |
121-06 | RVV Factor X Activator | 5 U | 1000.00 | Pentapharm |
121-07 | RVV-Factor X-Activator | 50 U | 6000.00 | Pentapharm |
502-01 | Pefakit® APC-R Factor V Leiden 3 x 40 tests | Kit | 3600.00 | Pentapharm |
502-21 | Pefakit® APC-R Factor V Leiden Controls | Kit | 1100.00 | Pentapharm |
505-01 | Pefakit® PiCT® | Kit | 4000.00 | Pentapharm |
505-22 | Pefakit® PiCT® Controls UFH | Kit | 3900.00 | Pentapharm |
800186 | Pefakit® TAFI | Kit | 10000.00 | Pentapharm |
800187 | Pefakit® TAFI Controls and Calibration | Kit | 6000.00 | Pentapharm |
800191 | Pefakit® Reptilase® Time | Kit | 2800.00 | Pentapharm |
069-03 | Prionex® 10% | 100 ml | 2000.00 | Pentapharm |
069-03 | Prionex® 10% | 500 ml | 9000.00 | Pentapharm |
069-03 | Prionex® 10% | 1000 ml | 12000.00 | Pentapharm |
801682 | Rabbit brain cephalin | 100mg | 12000.00 | Pentapharm |
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