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zeptometrix成立于1999年，是一家为药物研究行业提供诊断产品和服务的的私人控股公司。zeptometrix公司（ZMC）是一个传染病诊断行业先进者和创新者，为的客户提供值得信赖的产品和服务。zeptometrix是一个*集成的生物技术公司的产品支持的研发的所有阶段；发展，验证，制和商业化的诊断测试。zeptometrix不断开拓创新，致力为生命科学行业带来更多产品与服务。
Catalog #: 0801198
Bovine IgG ELISA
Preparation of Bovine IgG Standards
Tube Number | Concentration of Bovine IgG | Volume of Bovine IgG Standard | Volume of Assay Diluent |
1 | 125 ng/mL | 1000 ml | 0 ml |
2 | 62.5 ng/mL | 500 ml of #1 | 500 ml |
3 | 31.25 ng/mL | 500 ml of #2 | 500 ml |
4 | 15.6 ng/mL | 500 ml of #3 | 500 ml |
5 | 7.8 ng/mL | 500 ml of #4 | 500 ml |
6 | 0 ng/mL | 0 ml | 500 ml |
试验过程
使用前允许所有试剂达到室温。用于制备标准和样品的标号试管。如果不使用整个96井板，则取下剩余的井板。 从板框和放置到重新密封塑料袋与干燥剂。密封袋并存放在2-8°C。
步骤1：将每条标签贴在其末端标签上，以确保在检测过程中这些条带与板框分离时的标识。
步骤2：将标准1-6的200ml移入重复井.
步骤3：每个样品的移液管200 L成重复的威尔斯。
第四步：用盖板盖上盖板，在37°C下孵育30分钟。
第五步：抽取每口井的内容物，用1x板冲洗缓冲液冲洗4次。清洗时，用1倍平板冲洗缓冲液的300ml注满井，然后抽吸。执行4次填充/吸气循环 s.在后的清洗周期之后，仔细地在吸水性纸巾的垫子上仔细地敲打盘子，*地擦拭盘子。继续，直到没有观察到可见的液滴板洗缓冲液。
步骤6：将检测抗体的100m1移入各标准标本中。
不要将检测器抗体添加到底物空白井中。
步骤7：用盖板盖上盖板，在37°C下孵育30分钟。
步骤8：如步骤6所述，用板洗缓冲器冲洗4次。
步骤9：将基材的100m1移入每口井，包括基板空白井。
步骤10：室温下将盘子孵育30分钟。含有牛IgG的井中会出现蓝色。
步骤11：将100m1的止住溶液注入每口井。将发生从蓝色到黄色的颜色变化。
步骤12：在15分钟内，用微滴度平板阅读器读取450 nm处每口井的光密度。
试样稀释
牛初乳通常含有50~80 mg/mL的IgG抗体。1：1，000，000牛初乳稀释在试验稀释剂中被推荐用于初步测试。
牛乳通常含有0.5mg/mL的IgG抗体。建议在试验稀释液中稀释1：10，000的牛乳进行初步测试。
牛血清通常含有25-30mg/mL的IgG抗体。建议在试验稀释剂中稀释1：500，000牛血清进行初步测试。
程序流程图
制备试剂稀释剂
吸管标本和标准
37℃孵育30分钟
制水板
移液管检测器抗体
37℃孵育30分钟
制水板
移液管底液
室温孵育30分钟
添加停止溶液并在450 nm处读取
贮存
在2-8℃下储存所有试剂盒试剂。
不要冻结。
未使用的微量培养板应保存在带有干燥剂的密封袋中，以使暴露于水分降至低。
储存和处理的所有试剂均为ST 至少可以在包盒标签上打印的过期日期之前。
货号 | 品名 | 目录价 | 规格 | 品牌 |
0801539DNA-1UG | Cryptococcus Neoformans serotype D, DNA | 4524 | 1 ug | ZeptoMetrix |
0801538DNA-1UG | Candida Tropicalis Z012, DNA | 4524 | 1 ug | ZeptoMetrix |
0801544DNA-10UG | Proteus Mirabilis Z050, DNA | 7001 | 10 ug | ZeptoMetrix |
0801545DNA-10UG | Streptococcus Agalactiae 2603V - R, DNA | 7001 | 10 ug | ZeptoMetrix |
0801562DNA-10UG | Bacillus Subtilis Z094, DNA | 7001 | 10 ug | ZeptoMetrix |
0801563DNA-10UG | Citrobacter FreundII Z064, DNA | 7001 | 10 ug | ZeptoMetrix |
0801569DNA-10UG | Stenotrophomonas Maltophilia Z074, DNA | 7001 | 10 ug | ZeptoMetrix |
0801536DNA-1UG | Candida Krusei Z009, DNA | 4524 | 1 ug | ZeptoMetrix |
0801537DNA-1UG | Candida Parapsilosis Z011, DNA | 4524 | 1 ug | ZeptoMetrix |
0801535DNA-1UG | Candida Glabrata Z007, DNA | 4524 | 1 ug | ZeptoMetrix |
0801519DNA-10UG | Pseudomonas Aeruginosa clinical isolate, DNA | 7001 | 10 ug | ZeptoMetrix |
0801534DNA-10UG | Listeria Monocytogenes serotype 1 - 2b, DNA | 7001 | 10 ug | ZeptoMetrix |
0801518DNA-10UG | Enterobacter Aerogenes Z052, DNA | 7001 | 10 ug | ZeptoMetrix |
0801511DNA-10UG | Neisseria Meningitidis serogroup A, DNA | 7001 | 10 ug | ZeptoMetrix |
0801506DNA-10UG | Klebsiella Pneumoniae Z026, DNA | 7001 | 10 ug | ZeptoMetrix |
0801504DNA-1UG | Candida Albicans Z006, DNA | 4524 | 1 ug | ZeptoMetrix |
0801597DNA-10UG | Acinetobacter BaumannII 307-0294, DNA | 7001 | 10 ug | ZeptoMetrix |
0801579DNA-10UG | Mycoplasma Pneumoniae M129, DNA | 7001 | 10 ug | ZeptoMetrix |
0801679DNA-10UG | Haemophilus Influenzae type b; Eagan, DNA | 7001 | 10 ug | ZeptoMetrix |
0801676DNA-1UG | Histoplasma capsulatum Recombinant, DNA | 7001 | 1 ug | ZeptoMetrix |
0801677DNA-1UG | Coccidioides Immitis Recombinant, DNA | 7001 | 1 ug | ZeptoMetrix |
0801675DNA-10UG | Staphylococcus Aureus MSSA; mecA-deficient, DNA | 7001 | 10 ug | ZeptoMetrix |
0801674DNA-10UG | Yersinia Enterocolitica clinical isolate, DNA | 7001 | 10 ug | ZeptoMetrix |
0801651DNA-10UG | Staphylococcus Epidermidis MRSE; RP62A, DNA | 7001 | 10 ug | ZeptoMetrix |
0801650DNA-10UG | Campylobacter Jejuni clinical isolate, DNA | 7001 | 10 ug | ZeptoMetrix |
0801638DNA-10UG | Staphylococcus Aureus MRSA; COL, DNA | 7001 | 10 ug | ZeptoMetrix |
0801627DNA-10UG | Shigella Sonnei Z004, DNA | 7001 | 10 ug | ZeptoMetrix |
0801624DNA-10UG | Escherichia Coli ETEC; ST+, LT+ DNA | 2730 | 10 ug | ZeptoMetrix |
0801622DNA-10UG | Escherichia Coli O157:H7; EDL933, DNA | 2730 | 10 ug | ZeptoMetrix |
0801603DNA-1UG | Candida Lusitaniae Z010, DNA | 4524 | 1 ug | ZeptoMetrix |
0801619DNA-1UG | Clostridium Difficile NAP1, DNA | 7001 | 1 ug | ZeptoMetrix |
0801601DNA-1UG | Aspergillus Terreus Z016, DNA | 4524 | 1 ug | ZeptoMetrix |
0801602DNA-1UG | Candida GuilliermondII Z008, DNA | 4524 | 1 ug | ZeptoMetrix |
0801598DNA-1UG | Aspergillus Flavus Z013, DNA | 4524 | 1 ug | ZeptoMetrix |
0801680DNA-10UG | Haemophilus Influenzae type b; MinnA, DNA | 7001 | 10 ug | ZeptoMetrix |
0801482DNA-10UG | Neisseria Gonorrhoeae type strain, DNA | 7001 | 10 ug | ZeptoMetrix |
0801439DNA-10UG | Streptococcus Pneumoniae Z022; 19F (Danish), DNA | 7001 | 10 ug | ZeptoMetrix |
801223 | Goat IgG ELISA | 4875 | 96 det. | ZeptoMetrix |
801010 | Chicken IgY ELISA | 4875 | 96 det. | ZeptoMetrix |
801198 | Bovine IgG ELISA | 6689 | 96 det. | ZeptoMetrix |
801188 | ELISA Construction System | 5343 | 5 plates | ZeptoMetrix |
0801185L | ZeptoBlock | 3842 | 1 L | ZeptoMetrix |
0801185 | ZeptoBlock | 1697 | 120 ml | ZeptoMetrix |
0801184L | ZeptoBind | 3842 | 1 L | ZeptoMetrix |
0801184 | ZeptoBind | 839 | 60 ml | ZeptoMetrix |
0801179L | ZeptoCoat | 3842 | 1 L | ZeptoMetrix |
0801179 | ZeptoCoat | 839 | 60 ml | ZeptoMetrix |
0802001 | rhIL-2 Recominant Human Interleukin-2 | 5850 | 100 ug | ZeptoMetrix |
0801060 | 10x Plate Wash Buffer | 293 | 125 ml | ZeptoMetrix |
0801114 | rhIL-2 Recominant Human Interleukin-2 (20.000 units) | 1463 | 1.1 ml | ZeptoMetrix |
0801017 | TCGF (Natural T-Cell Growth Factor, IL-2) | 4700 | 50 ml | ZeptoMetrix |
0801195 | ZeptoGel | 5207 | 500 ml | ZeptoMetrix |
0801002 | HIV-1 p24 Antigen ELISA 2.0 Kit | 9341 | 96 det. | ZeptoMetrix |
0801221 | Horse IgG ELISA | 4875 | 96 det. | ZeptoMetrix |
0801008 | HIV-1 p24 Antigen ELISA 2.0 Kit | 38805 | 480 det. | ZeptoMetrix |
0801437DNA-10UG | Salmonella Enterica Typhimurium Z005, DNA | 7001 | 10 ug | ZeptoMetrix |
0801803P | Cryptococcus Neoformans Serotype A, antigen | 1092 | 1.0 ml | ZeptoMetrix |
0801202 | Rabbit IgG ELISA | 6689 | 96 det. | ZeptoMetrix |
0801009 | Rat IgG ELISA | 4875 | 96 det. | ZeptoMetrix |
0801181 | Mouse IgM ELISA | 6689 | 96 det. | ZeptoMetrix |
0801201 | SIV p27 Antigen ELISA Kit | 49764 | 480 det. | ZeptoMetrix |
0801200 | HIV-1 p24 Antigen ELISA Kit | 38805 | 480 det. | ZeptoMetrix |
0801197 | Human IgA ELISA | 6689 | 96 det. | ZeptoMetrix |
0801183 | Human IgM ELISA | 6689 | 96 det. | ZeptoMetrix |
0801182 | Human IgG ELISA | 6689 | 96 det. | ZeptoMetrix |
0801169 | SIV p27 Antigen ELISA Kit | 11993 | 96 det. | ZeptoMetrix |
0801137 | HIV-1 p24 Extended Range Kit | 1346 | kit | ZeptoMetrix |
0801116 | HTLV p19 Antigen ELISA Kit | 11993 | 96 det. | ZeptoMetrix |
0801111 | HIV-1 p24 Antigen ELISA Kit | 9341 | 96 det. | ZeptoMetrix |
0801096 | HIV-1 p24 ICx - CRx Kit | 1853 | 100 det. | ZeptoMetrix |
0801180 | Mouse IgG ELISA | 6689 | 96 det. | ZeptoMetrix |
0801693DNA-10UG | Enterococcus Faecalis VRE, DNA | 7001 | 10 ug | ZeptoMetrix |
0801084 | Anti-HTLV-I gp46 Clone 67 - 5.5.13.1 | 4388 | 100 ug | ZeptoMetrix |
0801119 | Anti-HTLV-II gp46 Clone 73 - 4.9.8 | 30732 | 1 mg | ZeptoMetrix |
0801120 | Anti-HTLV-II p24 Clone 75 - 4.21.11 | 30732 | 1 mg | ZeptoMetrix |
0801012 | Anti-PON1 Clone KRJ1 | 4388 | 100 ug | ZeptoMetrix |
0801014 | Anti-PON1 Clone KRJ2 | 4388 | 100 ug | ZeptoMetrix |
0801095 | Anti-PON1 Clone KRJ1 | 30732 | 1 mg | ZeptoMetrix |
0801097 | Anti-PON1 Clone KRJ2 | 30732 | 1 mg | ZeptoMetrix |
0801025 | Anti-Zika NS1 Clone 1D12 | 4388 | 100 ug | ZeptoMetrix |
0801032CFHI | HIV Type 1 Strain: IIIB - CFHI | 6045 | 1.0 ml | ZeptoMetrix |
0801033CFHI | Human T-Lymphotropic Virus Type I (HTLV-I) - CFHI | 6045 | 1.0 ml | ZeptoMetrix |
0801113 | Anti-HTLV-II p19 Clone 78 - 6.18.07 | 30732 | 1 mg | ZeptoMetrix |
0801093 | Anti-HTLV-II p19 Clone 78 - 6.18.07 | 4388 | 100 ug | ZeptoMetrix |
0801094 | Anti-HTLV-I gp46 Clone 65 - 6C2.2.34 | 30732 | 1 mg | ZeptoMetrix |
0801107 | Anti-HTLV-I p19 Clone TP-7 | 30732 | 1 mg | ZeptoMetrix |
0801108 | Anti-HTLV-I p19 Clone 45 - 6.11.1.3 | 30732 | 1 mg | ZeptoMetrix |
0801110 | Anti-HTLV-I p24 Clone 46 - 3.24.4 | 30732 | 1 mg | ZeptoMetrix |
0801127 | Anti-HTLV-I gp46 Clone 67 - 5.5.13.1 | 30732 | 1 mg | ZeptoMetrix |
0801118 | Anti-HTLV-I gp46 Clone 68 - 4.11.21 | 30732 | 1 mg | ZeptoMetrix |
0802004 | Anti-HTLV-I gp46 Clone 65 - 6C2.2.34 | 4388 | 100 ug | ZeptoMetrix |
0801086 | Anti-HTLV-II gp46 Clone 73 - 4.9.8 | 4388 | 100 ug | ZeptoMetrix |
0801087 | Anti-HTLV-II p24 Clone 75 - 4.21.11 | 4388 | 100 ug | ZeptoMetrix |
0801032 | Human Immunodeficiency Virus Type 1 (HIV-1) Strain: IIIB Lysate | 27593 | 1 mg | ZeptoMetrix |
0801033 | Human T-Lymphotropic Virus Type I (HTLV-I) Lysate | 31181 | 1 mg | ZeptoMetrix |
0801216 | Dinucleotide Standards ThymineGlycolCytidineDimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801217 | Dinucleotide Standards ThymineGlycolAdenineDimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801218 | Nucleoside Standards 8-Oxo-dG | 1287 | 1 mg | ZeptoMetrix |
0801219 | Nucleoside Standards 8-Oxo-dA | 1365 | 1 mg | ZeptoMetrix |
0801384 | PON Standards | 5265 | 600 ul | ZeptoMetrix |
0801500 | Simian Immunodeficiency Virus (SIV) Kit (10 Strip) | 9360 | 10 det. | ZeptoMetrix |
0801501 | Simian Immunodeficiency Virus (SIV) Kit (30 Strip) | 22854 | 30 det. | ZeptoMetrix |
0801502 | Simian Immunodeficiency Virus (SIV) Strips (10/tube) | 5343 | 10 strips | ZeptoMetrix |
0801021 | Zika (PRVABC59) Strips (10/tube) | 5343 | 10 strips | ZeptoMetrix |
0801215 | Dinucleotide Standards ThymineGlycolThymineDimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801214 | Dinucleotide Standards ThymineGlycolGuanineDimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801808 | Scedosporium prolificans Z083 | 8775 | 1.0 ml | ZeptoMetrix |
0801192 | TBARS Assay kit | 7995 | 160 det. | ZeptoMetrix |
0801199 | Arylesterase - Paraoxonase Assay Kit | 7995 | 200 tests | ZeptoMetrix |
0805002 | Total Glutathione Peroxidase Assay Kit | 7625 | 100 tests | ZeptoMetrix |
0805004 | Glutathione Reductase Assay Kit | 10257 | 100 tests | ZeptoMetrix |
0801210 | Dinucleotide Standards Formamido Guanine Dimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801211 | Dinucleotide Standards Formamido Cytidine Dimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801212 | Dinucleotide Standards Formamido Thymine Dimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801213 | Dinucleotide Standards Formamido Adenine Dimer | 1287 | 50 mg at 1.0 OD | ZeptoMetrix |
0801022 | Zika (MR-766) Strips (10/tube) | 5343 | 10 strips | ZeptoMetrix |
0801085 | Anti-HTLV-I gp46 Clone 68 - 4.11.21 | 4388 | 100 ug | ZeptoMetrix |
0801698DNA-1UG | Pneumocystis Jiroveci Recombinant W303-Pji, Genomic DNA | 7001 | 10 ug | ZeptoMetrix |
0801765DNA-1UG | Scedosporium Apiospermum Z085, DNA | 4524 | 1 ug | ZeptoMetrix |
0801770DNA-1UG | Fusarium Oxysporum Z084, DNA | 4524 | 1 ug | ZeptoMetrix |
0801802DNA-1UG | Tremella Fuciformis Recombinant, DNA | 7001 | 1 ug | ZeptoMetrix |
0801806DNA-1UG | Fusarium solani Z081, DNA | 4524 | 1 ug | ZeptoMetrix |
0801808DNA-1UG | Scedosporium Prolificans Z083, DNA | 4524 | 1 ug | ZeptoMetrix |
0801827DNA-1UG | Aspergillus Niger Z105, DNA | 4524 | 1 ug | ZeptoMetrix |
0801834DNA-1UG | Rhizopus oryzae Z106, DNA | 4524 | 1 ug | ZeptoMetrix |
0801899DNA-10UG | Plesiomonas shigelloides Z130, DNA | 7001 | 10 ug | ZeptoMetrix |
0801901DNA-10UG | Vibrio Cholerae Z132 (toxigenic) | 7001 | 10 ug | ZeptoMetrix |
0801761DNA-1UG | Mucor Indicus Z079, DNA | 4524 | 1 ug | ZeptoMetrix |
0801759DNA-1UG | Cunninghamella bertholletiae Z080, DNA | 4524 | 1 ug | ZeptoMetrix |
0801700DNA-1UG | Cryptosporidium Parvum-S. cerevisiae Recombinant, DNA | 7001 | 1.0 ml | ZeptoMetrix |
0801716DNA-1UG | Aspergillus Fumigatus Z014, DNA | 4524 | 1 ug | ZeptoMetrix |
0801723DNA-10UG | Serratia Marcescens Z053, DNA | 7001 | 10 ug | ZeptoMetrix |
0801697DNA-1UG | Blastomyces Dermatitidis Recombinant W303-Bde, Genomic DNA | 7001 | 1 ug | ZeptoMetrix |
0801736DNA-10UG | Haemophilus ducreyi class I, DNA | 7001 | 10 ug | ZeptoMetrix |
0801743DNA-10UG | Bacteroides Thetaiotaomicron Z037 DNA | 7001 | 10 ug | ZeptoMetrix |
0801745DNA-10UG | Citrobacter Koseri Z039, DNA | 7001 | 10 ug | ZeptoMetrix |
0801747DNA-10UG | Escherichia coli O111:NM, DNA | 7001 | 10 ug | ZeptoMetrix |
0801755DNA-1UG | Rhizopus Microsporus Z056, DNA | 4524 | 1 ug | ZeptoMetrix |
0801004 | Anti-HIV-l p24 Clone 38 - 8.7.47 | 4388 | 100 ug | ZeptoMetrix |
0801005 | Anti-HIV-l p17 Clone 32 - 5.8.42 | 4388 | 100 ug | ZeptoMetrix |
0801124 | Anti-HIV-l p24 Clone 32 - 5.17.76 | 30732 | 1 mg | ZeptoMetrix |
0801125 | Anti-HIV-l p24 Clone 38 - 8.7.47 | 30732 | 1 mg | ZeptoMetrix |
0801126 | Anti-HIV-l p24 Clone 39 - 5.1.23 | 30732 | 1 mg | ZeptoMetrix |
0801128 | Anti-HIV-l RT Clone 39 - 4.12.2 | 30732 | 1 mg | ZeptoMetrix |
0801136 | Anti-HIV-l p24 Clone 39 - 5.4A | 30732 | 1 mg | ZeptoMetrix |
0801003 | Anti-HTLV-I p19 Clone TP-7 | 4388 | 100 ug | ZeptoMetrix |
0802014 | Anti-HIV-l p24 Clone 39 - 6.14 | 30732 | 1 mg | ZeptoMetrix |
0801018 | Anti-HTLV-I p24 Clone 46 - 3.24.4 | 4388 | 100 ug | ZeptoMetrix |
0801082 | Anti-HTLV-I p19 Clone 45 - 6.11.1.3 | 4388 | 100 ug | ZeptoMetrix |
0801123 | Anti-HIV-l p17 Clone 32 - 5.8.42 | 30732 | 1 mg | ZeptoMetrix |
0801122 | Anti-HIV-l p17 Clone 2D11 | 30732 | 1 mg | ZeptoMetrix |
0801121 | Anti-HIV-l gp41 Clone 10E9 | 30732 | 1 mg | ZeptoMetrix |
0801006 | Anti-HIV-l gp41 Clone 10E9 | 4388 | 100 ug | ZeptoMetrix |
0801007 | Anti-HIV-l RT Clone 39 - 4.12.2 | 4388 | 100 ug | ZeptoMetrix |
0801076 | Anti-HIV-l p17 Clone 2D11 | 4388 | 100 ug | ZeptoMetrix |
0801077 | Anti-HIV-l p17 Clone 32 - 1.24.89 | 4388 | 100 ug | ZeptoMetrix |
0801078 | Anti-HIV-l p24 Clone 32 - 5.17.76 | 4388 | 100 ug | ZeptoMetrix |
0801079 | Anti-HIV-l p24 Clone 39 - 5.1.23 | 4388 | 100 ug | ZeptoMetrix |
0801080 | Anti-HIV-l p24 Clone 39 - 5.4A | 4388 | 100 ug | ZeptoMetrix |
0801081 | Anti-HIV-l p24 Clone 39 - 6.14 | 4388 | 100 ug | ZeptoMetrix |
0801115 | Anti-HIV-l p17 Clone 32 - 1.24.89 | 30732 | 1 mg | ZeptoMetrix |
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