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GBI Labs / Golden Bridge International（GBI），Inc。成立于1993年，旨在为世界各地的研究，临床，生物技术和制药实验室提供*，经济，创新的病理学试剂和试剂盒。今天，GBI实验室继续开发新产品，并制造高质量的免疫组织化学（IHC）试剂，用于临床和非临床研究中癌症和其他疾病的抗原/蛋白质筛查; 基于抗体的药物发现：和人类解剖病理学和兽医病理学中的传统应用蛋白质/抗体筛选。
GBI Labs*的系列包括病理学辅助试剂，具有辣根过氧化物酶[HRP]和碱性磷酸酶[AP]聚合物技术的检测试剂盒，以及除传统小鼠，兔，大鼠和山羊外的多种宿主物种的检测。我们拥有多的双染色和三染色试剂盒，其中含有色原，可以使用光学显微镜对共定位蛋白表达进行半定量分析。我们的Klear Human试剂盒可在没有背景的情况下快速检测人体组织上的人源或人源化抗体。我们为其他物种提供Klear试剂盒，如小鼠，兔子和大鼠。
特征
专门设计用于检测人体组织上的人IgG抗体，背景干净。
该聚合物检测系统吸附于人血清蛋白，以获得优异的特异性和灵敏度。
一如既往，GBI聚合物试剂盒不含生物素，可节省额外的阻断步骤。
简单的快速协议
将引物和人一抗在4 ℃ 温育过夜。
将人1'Ab混合物在组织上孵育（30分钟至2小时）
将抗人聚合物-HRP缀合物孵育10分钟。
5至20分钟的色原培养。选择色原试剂盒：DAB，DAB-Ni，AEC，Permanent Red，Fast-Red，BCIP / NBT，AP-Blue。
Klear Human HRP-Polymer DAB Detection System
核人HRP-聚合物民建联检测系统
预定用途：
采用间接检测法时，用同一种属的一次抗体进行抗原检测，可产生较高的背景值。这严重限制了筛查人抗体的使用。 在人体组织上。GBI实验室KlearHumanHRP用于民建联-聚合物检测系统设计，用于在人体组织上无背景染色的人原抗体染色。核人HRP-聚合物 检测试剂盒提供特殊的阻断缓冲液，聚合HRP连接的二次抗体以及人的引物在一个现成的使用系统。这项技术需要与pri进行通宵的预孵化。 玛丽抗体具有良好的敏感性和高特异性。它是一种不含生物素的系统，克服了由链霉亲和素/生物素系统引起的非特异性染色。 奥斯生物素。注：本试剂盒适用于细胞质和膜结合抗原，其他染色模式尚未检测。
组件
Component No. | Content | 6mL Kit | 18mL Kit | 110mL Kit |
Reagent 1 | Human Primer (RTU) | 6mL | 18mL | 110 mL |
Reagent 2 | Quenching Buffer (5xConcentrate) | 1.5mL | 2.3mLx2 | 13 mL x2 |
Reagent 3 | Hu Blocking A (RTU) | 6mL | 18mL | 110 mL |
Reagent 4 | Hu Blocking B (RTU) | 6mL | 18mL | 110 mL |
Reagent 5 | Human HRP Polymer (RTU) | 6mL | 18mL | 110 mL |
Reagent 6A | DAB Substrate (RTU) | 12mL | 2x15mL | 120 mL |
Reagent 6B | DAB Chromogen (20x) | 1.5mL | 2mL | 6 mL |
建议的议定书：
固定：为了保证染色的质量和获得可复制的性能，用户需要提供适当的固定组织和准备好的幻灯片。
组织需要紧紧粘在滑块上，以避免组织脱落。
石蜡包埋切片在染色前必须先用二甲苯进行脱粘，再用分级的乙醇进行再水化。
细胞涂片应尽量制成单层，以获得满意的结果。
三种对照幻灯片将有助于解释结果：阳性组织控制，试剂控制(用等型对照试剂处理)，和阴性对照。
开始染色程序：不要让标本或组织从这一点开始干燥。
议定书说明：
固定、组织切片厚度、抗原提取、一次抗体稀释和孵育时间效应均有显着性差异(P<0.05)。调查人员需要考虑所有因素并确定宜方案。 解释结果时的条件。
组织染色取决于染色前组织的正确处理和处理。不适当的组织准备可能导致假阴性结果或不一致的结果。
不要混合不同批次的试剂。
在染色过程中，不要让幻灯片在任何时候干燥。
相关产品：
Product | Catalog No. | Size |
Klear Mouse AP Fast Red kit | D50-6 / D50-18 | 6ml / 18ml |
Klear Mouse HRP with AEC Kit | D53-6 / D53-18 | 6ml / 18ml |
Klear Mouse AP AP-Red Kit | D51-6 / D51-18 | 6ml / 18ml |
Klear Mouse Blocking A & B | D54-100 / D54-18 | 100ml / 18ml |
Klear Rat HRP DAB kit | D98-6 / D98-18 | 6ml / 18ml |
Klear Rat HRP AEC kit | D99-6 / D99-18 | 6ml / 18ml |
Klear Rat AP Fast-Red | D100-6 / D100-18 | 6ml / 18ml |
Klear Rat AP AP-red | D101-6 / D101-18 | 6ml / 18ml |
Klear Rat Blocking A & B | D102-18 | 18ml |
Polink-2 Plus HRP RAT-NM DAB kit for Rat antibody on Mouse Tissue | D46-6 / D46-18 | 6ml / 18ml |
Polink-2 Plus HRP RAT-NM AEC kit for Rat antibody on Mouse Tissue | D48-6 / D48-18 | 6ml / 18ml |
Polink-2 Plus AP RAT-NM kit for Rat antibody on Mouse Tissue | D67-18 / D67-6 | 6ml / 18ml |
Polink-2 Plus HRP Mouse-NR DAB kit for Mouse antibody on Rat tissue | D58-6 / D58-18 | 6ml / 18ml |
Polink-2 Plus HRP Mouse-NR AEC kit for Mouse antibody on Rat tissue | D59-6 / D59-18 | 6ml / 18ml |
Polink-2 Plus AP Mouse-NR kit for Mouse antibody on Rat tissue | D65-18 / D65-6 | 6ml / 18ml |
警惕
当处理任何或所有试剂时，应将所有组件作为潜在危险材料处理，请戴手套、保护眼睛、适当的实验室整体(除实验室外套外)。
话语( remark的名词复数 ):
只作研究用途
货号 | 品名 | 规格 | |
D103-110D | Klear Human HRP-Polymer DAB Bulk with DAB Kit | 110mL | GBI Labs |
D103-18 | Klear Human HRP-Polymer DAB Kit | 18ml | GBI Labs |
D103-6 | Klear Human HRP-Polymer DAB Kit | 6ml | GBI Labs |
D104-18 | Klear Human HRP-polymer with AEC Kit | 18ml | GBI Labs |
D104-6 | Klear Human HRP-polymer with AEC Kit | 6ml | GBI Labs |
D105-18 | Klear Human AP-polymer with Fast Red Kit | 18ml | GBI Labs |
D105-6 | Klear Human AP-polymer with Fast Red Kit | 6ml | GBI Labs |
D106-18 | Klear Human AP-polymer with Permanent Red Kit | 18ml | GBI Labs |
D106-6 | Klear Human AP-polymer with Permanent Red Kit | 6ml | GBI Labs |
D27-110 | Polink-2 AP Broad-3 (Ms. Rb. Gt) Bulk Kit | 110ml | GBI Labs |
D27-18/D27-6 | Polink-2 AP Broad-3 (Ms. Rb. Gt) with Permanent Red Kit | 18ml/6ml | GBI Labs |
D86-18/D86-6 | Polink-2 Plus HRP Syrian Hamster with DAB Kit | 18ml/6ml | GBI Labs |
D86-60 | Polink-2 Plus HRP Syrian Hamster for DAB Bulk Kit | 60ml | GBI Labs |
D22-110/D22-60 | Polink-2 HRP Broad for DAB Bulk Kit | 110ml/60ml | GBI Labs |
D22-18/D22-6 | Polink-2 HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D22-60D | Polink-2 HRP Broad with DAB Kit | 60mL | GBI Labs |
D23-110/D23-60 | Polink-2 HRP Broad for AEC Bulk Kit | 110ml/60ml | GBI Labs |
D23-18/D23-6 | Polink-2 HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D24-110 | Polink-2 AP Broad Bulk Kit | 110ml | GBI Labs |
D24-18/D24-6 | Polink-2 AP Broad with Permanent Red Kit | 18ml/6ml | GBI Labs |
D11-110 | Polink-1 HRP Broad for DAB Bulk Kit | 110ml | GBI Labs |
D11-18/D11-6 | Polink-1 HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D11-60 | Polink-1 HRP Broad for DAB Bulk Kit | 60mL | GBI Labs |
D14-110 | Polink-1 HRP Broad for AEC Bulk Kit | 110ml | GBI Labs |
D14-18/D14-6 | Polink-1 HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D14-60 | Polink-1 HRP Broad for AEC Bulk Kit | 60mL | GBI Labs |
D17-110 | Polink-1 AP Broad Bulk Kit | 110ml | GBI Labs |
D17-18/D17-6 | Polink-1 AP Broad with Permanent Red Kit | 18ml/6ml | GBI Labs |
D41-110 | Polink-2 Plus HRP Broad for DAB Bulk Kit | 110ml | GBI Labs |
D41-125 | Polink-2 Plus HRP Broad with DAB Bulk Kit | 125ml | GBI Labs |
D41-18/D41-6 | Polink-2 Plus HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D42-110 | Polink-2 Plus HRP Broad for AEC Bulk Kit | 110ml | GBI Labs |
D42-18/D42-6 | Polink-2 Plus HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D68-110 | Polink-2 Plus AP Broad Bulk Kit | 110ml | GBI Labs |
D68-18/D68-6 | Polink-2 Plus AP Broad Kit | 18ml/6ml | GBI Labs |
D01(2)-110/D01(2)-18 | Biotin-2nd Ab Mouse & Rabbit | 110mL/18mL | GBI Labs |
D01-110/D01-60 | SPlink HRP Broad Bulk Kit | 110ml/60ml | GBI Labs |
D01-125 | SPlink HRP Broad Bulk Kit | 125mL | GBI Labs |
D01-18/D01-6 | SPlink HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D04-18/D04-6 | SPlink HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D07-110/ D07-60 | APlink AP Broad Bulk Kit | 110ml/60ml | GBI Labs |
D07-18A/D07-6A | APlink AP Broad with AP-Red+ Kit | 18ml/6ml | GBI Labs |
D07-18F/D07-6F | APlink AP Broad with Fast Red Kit | 18ml/6ml | GBI Labs |
D25-18 | Streptavidin-HRP 18ml kit (no 2nd antibody provided) | 18ml | GBI Labs |
D25-500/D25-110 | Streptavidin-HRP Bulk Kit (no 2nd antibody provided) | 500ml/110ml | GBI Labs |
D29-110 | Streptavidin-AP Bulk Kit (no 2nd antibody provided) | 110ml | GBI Labs |
D29-18 | Streptavidin-AP 18ml kit (no 2nd antibody provided) | 18ml | GBI Labs |
D12-110 | Polink-1 HRP Mouse for DAB Bulk Kit | 110ml | GBI Labs |
D12-18/D12-6 | Polink-1 HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D12-60 | Polink-1 HRP Mouse for DAB Bulk Kit | 60mL | GBI Labs |
D12-60D | Polink-1 HRP Mouse for DAB Bulk Kit | 60mL | GBI Labs |
D15-110 | Polink-1 HRP Mouse for AEC Bulk Kit | 110ml | GBI Labs |
D15-18/D15-6 | Polink-1 HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D18-110 | Polink-1 AP Mouse Bulk Kit | 110ml | GBI Labs |
D18-18/D18-6 | Polink-1 AP Mouse with Permanent Red Kit | 18ml/6ml | GBI Labs |
D37-110 | Polink-2 Plus HRP Mouse for DAB Bulk Kit | 110ml | GBI Labs |
D37-18/D37-6 | Polink-2 Plus HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D38-110 | Polink-2 Plus HRP Mouse for AEC Bulk Kit | 110ml | GBI Labs |
D38-18/D38-6 | Polink-2 Plus HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D69-110 | Polink-2 Plus AP Mouse Bulk Kit | 110ml | GBI Labs |
D69-18/D69-6 | Polink-2 Plus AP Mouse with Permanent Red Kit | 18ml/6ml | GBI Labs |
D02(2)-110 | Biotin-2nd Ab Mouse | 110mL | GBI Labs |
D02(2)-18 | Biotin-2nd Ab Mouse | 18mL | GBI Labs |
D02-110 | SPlink HRP Mouse Bulk Kit | 110ml | GBI Labs |
D02-18/D02-6 | SPlink HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D02-500 | SPlink HRP Mouse Bulk Kit | 500mL | GBI Labs |
D05-18/D05-6 | SPlink HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D08-110 | APlink AP Mouse Bulk Kit | 110ml | GBI Labs |
D08-18A/D08-6A | APlink AP Mouse with Permanent Red Kit | 18ml/6ml | GBI Labs |
D08-18F/D08-6F | APllink AP Mouse with Fast Red Kit | 18ml/6ml | GBI Labs |
D31-1 | Simplified Streptavidin-HRP Mouse Concentrate (100x) | 1ml | GBI Labs |
D13-110 | Polink-1 HRP Rabbit for DAB Bulk Kit | 110ml | GBI Labs |
D13-18/D13-6 | Polink-1 HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D16-110 | Polink-1 HRP Rabbit for AEC Bulk Kit | 110ml | GBI Labs |
D16-18/D16-6 | Polink-1 HRP Rabbit with AEC Kit | 18ml/6ml | GBI Labs |
D19-110 | Polink-1 AP Rabbit Bulk Kit | 110ml | GBI Labs |
D19-18/D19-6 | Polink-1 AP Rabbit with Permanent Red Kit | 18ml/6ml | GBI Labs |
D39-110 | Polink-2 Plus HRP Rabbit for DAB Bulk Kit | 110ml | GBI Labs |
D39-18/D39-6 | Polink-2 Plus HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D40-110 | Polink-2 Plus HRP Rabbit for AEC Bulk Kit | 110ml | GBI Labs |
D40-18/D40-6 | Polink-2 Plus HRP Rabbit with AEC Kit | 18ml/6ml | GBI Labs |
D70-110 | Polink-2 Plus AP Rabbit Bulk Kit | 110ml | GBI Labs |
D70-18/D70-6 | Polink-2 Plus AP Rabbit with Permanent Red Kit | 18ml/6ml | GBI Labs |
D03(2)-110 | Biotin-2nd Ab Rabbit | 110mL | GBI Labs |
D03(2)-18 | Biotin-2nd Ab Rabbit | 18mL | GBI Labs |
D03-110 | SPlink HRP Rabbit Bulk Kit | 110ml | GBI Labs |
D03-18/D03-6 | SPlink HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D51-18/D51-6 | Klear Mouse AP with Permanent Red Kit | 18ml/6ml | GBI Labs |
D52-110 | Klear Mouse HRP for DAB Bulk Kit | 110ml | GBI Labs |
D52-18/D52-6 | Klear Mouse HRP with DAB Kit | 18ml/6ml | GBI Labs |
D53-110 | Klear Mouse HRP for AEC Bulk Kit | 110ml | GBI Labs |
D53-18/D53-6 | Klear Mouse HRP with AEC Kit | 18ml/6ml | GBI Labs |
D54-100 | Klear Mouse Blocking Buffer | 100mL | GBI Labs |
D54-18/D54-6 | Klear Mouse Blocking Buffer | 18mL/6mL | GBI Labs |
D55-110 | Polink-1 HRP Mouse-NR for DAB Bulk Kit | 110ml | GBI Labs |
D55-18/D55-6 | Polink-1 HRP Mouse-NR with DAB Kit | 18ml/6ml | GBI Labs |
D56-110 | Polink-1 HRP Mouse-NR for AEC Bulk Kit | 110ml | GBI Labs |
D56-18/D56-6 | Polink-1 HRP Mouse-NR with AEC Kit | 18ml/6ml | GBI Labs |
D57-110 | Polink-1 AP Mouse-NR Bulk Kit | 110ml | GBI Labs |
D57-18/D57-6 | Polink-1 AP Mouse-NR Kit | 18ml/6ml | GBI Labs |
D58-110 | Polink-2 Plus HRP Mouse-NR for DAB Bulk Kit | 110ml | GBI Labs |
D58-18/D58-6 | Polink-2 Plus HRP Mouse-NR with DAB Kit | 18ml/6ml | GBI Labs |
D59-110 | Polink-2 Plus HRP Mouse-NR for AEC Bulk Kit | 110ml | GBI Labs |
D59-18 /D59-6 | Polink-2 Plus HRP Mouse-NR with AEC Kit | 18ml/6ml | GBI Labs |
D65-110 | Polink-2 Plus AP Mouse-NR Bulk Kit | 110ml | GBI Labs |
D65-18/D65-6 | Polink-2 Plus AP Mouse-NR Kit | 18ml/6ml | GBI Labs |
D12-110 | Polink-1 HRP Mouse for DAB Bulk Kit | 110ml | GBI Labs |
D12-18/D12-6 | Polink-1 HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D15-110 | Polink-1 HRP Mouse for AEC Bulk Kit | 110ml | GBI Labs |
D15-18/D15-6 | Polink-1 HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D18-110 | Polink-1 AP Mouse Bulk Kit | 110ml | GBI Labs |
D18-18/D18-6 | Polink-1 AP Mouse with GBI-Permanent Red Kit | 18ml/6ml | GBI Labs |
上海起发实验试剂有限公司是实验试剂一站式采购服务商
1：*的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。
2：产品种类齐全，经营超过700多品牌，基本涵盖所有生物实验试剂耗材。
3：提供加急服务，货品一般1-2周到货。
4：富有竞争力的价格优势，绝大部分价格有优势。
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6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司授权代理。
7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发，欢迎合作。
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