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GBI Labs / Golden Bridge International（GBI），Inc。成立于1993年，旨在为世界各地的研究，临床，生物技术和制药实验室提供*，经济，创新的病理学试剂和试剂盒。今天，GBI实验室继续开发新产品，并制造高质量的免疫组织化学（IHC）试剂，用于临床和非临床研究中癌症和其他疾病的抗原/蛋白质筛查; 基于抗体的药物发现：和人类解剖病理学和兽医病理学中的传统应用蛋白质/抗体筛选。
GBI Labs*的系列包括病理学辅助试剂，具有辣根过氧化物酶[HRP]和碱性磷酸酶[AP]聚合物技术的检测试剂盒，以及除传统小鼠，兔，大鼠和山羊外的多种宿主物种的检测。我们拥有多的双染色和三染色试剂盒，其中含有色原，可以使用光学显微镜对共定位蛋白表达进行半定量分析。我们的Klear Human试剂盒可在没有背景的情况下快速检测人体组织上的人源或人源化抗体。我们为其他物种提供Klear试剂盒，如小鼠，兔子和大鼠。
特征
一步聚合物检测系统，灵敏度高。不含生物素。
检测小鼠IgG
孵化15分钟。
人和兔血清蛋白被吸附。
选择色原试剂盒：DAB，DAB-Ni，AEC，Permanent Red，Fast-Red，BCIP / NBT，AP-Blue
Polink-1 HRP Detection System for Mouse Antibody (for DAB) (Polymer-HRP detection system, biotin-free, Anti-mouse) Ready-to-use One Step Polymer Detection System
Polink-1小鼠抗体HRP检测系统
（聚合物HRP检测系统，无生物素，抗小鼠）
一步法聚合物检测系统
Storage: 4-8ºC
Catalog No. D12-110 110 ml (bulk, w/o chromogen)
D12-60 60ml (bulk, w/o chromogen)
D12-18 18 ml (with DAB, good for 180 slides) D12-6 6 ml (with DAB, good for 50 slides)
预定用途：
目录号D12110 110毫升（散装，W/O显色剂）
D12-60毫升(体积，w/o色原)
d12-18 18毫升(dda，好180张幻灯片)d12-6 6毫升(dda，好50张幻灯片)
Polink-1 HRP小鼠民建联检测试剂盒的目的是利用用户提供的小鼠抗体在人体组织或细胞标本上检测靶抗原。标本可以冷冻或石蜡包埋的组织， 新制备的单层细胞涂片。
POLIKI-1 1HRP小鼠DAB检测试剂盒是一种使用聚合辣根过氧化物酶（HRP）的一步聚合物检测系统。
-链接山羊抗鼠IgG，直接检测与组织结合的初级抗体。该技术具有良好的敏感性和高特异性。因此，这是一种无生物素的系统。 由内源性生物素1引起链霉亲和素/生物素系统引起的非特异性染色。这是一个一步检测系统，比传统的两步检测方法要快得多。 生物素化的第二抗体，然后是链霉亲和素-HRP)。AEC Stai 宁选择POLIK-1 HRP小鼠为AEC（D15 110、D15 18、D15～6）。
Catalog No. | Product Name | Reagent 1: Polymer HRP-linked anti-mouse IgG (Ready-to-use) | Reagent 2: 2A: DAB Substrate 2B: Chromogen concentrate |
D12-110 | Polink-1 HRP Mouse Bulk for DAB kit | 110ml | Not provided |
D12-60 | Polink-1 HRP Mouse Bulk for DAB kit | 60ml | Not provided |
D12-18 | Polink-1 HRP Mouse DAB kit | 18ml | 30 ml of 2A and 2 ml of 2B |
D12-6 | Polink-1 HRP Mouse DAB kit | 6ml | 12 ml of 2A and 1.5 ml of 2B |
Reagent | Staining Procedure | Incubation Time (Min.) |
1. Peroxidase Blocking Reagent Supplied by user | a. Incubate slides in peroxidese blocking reagent (Ready-to-use 3% H2O2 solution) for 10 min. b. Rinse the slide using distilled water. | 10 |
2. HIER Pretreatment: Refer to antibody data sheet. | a. Heat Induced Epitope Retrieval (HIER) may be required for primary antibody suggested by vendor. b. Wash with PBS 3 times for 2 minutes each time. | Refer to vendor’s data sheet |
3. Pre-Block (Optional) Not provided | a. Add 2 (100 μL) or more drops of 10% Normal Goat Serum to cover the tissue section and Incubate 10 min. b. Drain or blot off solution. DO NOT RINSE. | 10 |
4. Primary antibody: Supplied by user | Notes: Investigator needs to optimize dilution and incubation times a. Apply 2 (100 μL) or more drops of primary antibody to cover the tissue completely. Incubate in moist chamber for 30-60 min. b. Rinse with PBS containing 0.05% Tween-20 3 times for 2 minutes each time. | 30-60 |
5. Reagent 1: HRP Polymer-anti-Mouse IgG | a. Apply 2 (100 μL) or more drops of HRP Polymer-anti-Mouse IgG to cover tissue section and Incubate in moist chamber for 10-15 min. | 15 |
建议的议定书：
固定：为了保证染色的质量和获得可复制的性能，用户需要提供适当的固定组织和准备好的幻灯片。
组织需要紧紧粘在滑块上，以避免组织脱落。
石蜡包埋切片在染色前必须先用二甲苯进行脱粘，再用分级的乙醇进行再水化。
细胞涂片应尽量多做单层，以获得满意的结果。
研究人员需要优化原抗体的稀释和孵育时间。
三种对照幻灯片将有助于解释结果：阳性组织控制，试剂控制(用等型控制试剂处理的幻灯片)和阴性对照。
进行IHC染色：不要让标本或组织从这一点开始干燥。
议定书说明：
固定时间、组织切片厚度、一抗体稀释度和孵育时间对结果有显著影响。调查人员需要考虑所有因素，并在下列情况下确定宜条件： 解释结果。
组织染色取决于染色前组织的正确处理和处理。不适当的组织准备可能导致假阴性结果或不一致的结果。
不要混合不同批次的试剂。
在染色过程中，不要让幻灯片在任何时候干燥。
＊POLIK-1 HRP大鼠NIT试剂盒与小鼠原代抗体不交叉反应
*Polink-1 HRP小鼠-NR试剂盒不与大鼠一次抗体发生交叉反应。
有防备的，戒备的，警惕的:
请戴上手套，并采取其他必要的预防措施。
话语( remark的名词复数 ):
只供研究用。
参考( reference的名词复数 ):
Bisgaard K，PluizedKP.聚合物结合物在免疫化学中的应用：传统染色方法与利用聚合物共轭物染色方法的比较研究。21 Intl Cong Intl Acad Pathol和12 World Cong Acad Environ Pathol.布达佩斯，饥饿，1996年10月20-25日。
石泽。Itzkowitz SH，Kim YS.三种免疫过氧化物酶技术检测结直肠癌组织抗原的比较j Hitochem Cytochem 36：317-322，
GBI Labs 2018年产品列表：
货号 | 品名 | 规格 | 品牌 |
D103-110D | Klear Human HRP-Polymer DAB Bulk with DAB Kit | 110mL | GBI Labs |
D103-18 | Klear Human HRP-Polymer DAB Kit | 18ml | GBI Labs |
D103-6 | Klear Human HRP-Polymer DAB Kit | 6ml | GBI Labs |
D104-18 | Klear Human HRP-polymer with AEC Kit | 18ml | GBI Labs |
D104-6 | Klear Human HRP-polymer with AEC Kit | 6ml | GBI Labs |
D105-18 | Klear Human AP-polymer with Fast Red Kit | 18ml | GBI Labs |
D105-6 | Klear Human AP-polymer with Fast Red Kit | 6ml | GBI Labs |
D106-18 | Klear Human AP-polymer with Permanent Red Kit | 18ml | GBI Labs |
D106-6 | Klear Human AP-polymer with Permanent Red Kit | 6ml | GBI Labs |
D27-110 | Polink-2 AP Broad-3 (Ms. Rb. Gt) Bulk Kit | 110ml | GBI Labs |
D27-18/D27-6 | Polink-2 AP Broad-3 (Ms. Rb. Gt) with Permanent Red Kit | 18ml/6ml | GBI Labs |
D86-18/D86-6 | Polink-2 Plus HRP Syrian Hamster with DAB Kit | 18ml/6ml | GBI Labs |
D86-60 | Polink-2 Plus HRP Syrian Hamster for DAB Bulk Kit | 60ml | GBI Labs |
D22-110/D22-60 | Polink-2 HRP Broad for DAB Bulk Kit | 110ml/60ml | GBI Labs |
D22-18/D22-6 | Polink-2 HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D22-60D | Polink-2 HRP Broad with DAB Kit | 60mL | GBI Labs |
D23-110/D23-60 | Polink-2 HRP Broad for AEC Bulk Kit | 110ml/60ml | GBI Labs |
D23-18/D23-6 | Polink-2 HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D24-110 | Polink-2 AP Broad Bulk Kit | 110ml | GBI Labs |
D24-18/D24-6 | Polink-2 AP Broad with Permanent Red Kit | 18ml/6ml | GBI Labs |
D11-110 | Polink-1 HRP Broad for DAB Bulk Kit | 110ml | GBI Labs |
D11-18/D11-6 | Polink-1 HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D11-60 | Polink-1 HRP Broad for DAB Bulk Kit | 60mL | GBI Labs |
D14-110 | Polink-1 HRP Broad for AEC Bulk Kit | 110ml | GBI Labs |
D14-18/D14-6 | Polink-1 HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D14-60 | Polink-1 HRP Broad for AEC Bulk Kit | 60mL | GBI Labs |
D17-110 | Polink-1 AP Broad Bulk Kit | 110ml | GBI Labs |
D17-18/D17-6 | Polink-1 AP Broad with Permanent Red Kit | 18ml/6ml | GBI Labs |
D41-110 | Polink-2 Plus HRP Broad for DAB Bulk Kit | 110ml | GBI Labs |
D41-125 | Polink-2 Plus HRP Broad with DAB Bulk Kit | 125ml | GBI Labs |
D41-18/D41-6 | Polink-2 Plus HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D42-110 | Polink-2 Plus HRP Broad for AEC Bulk Kit | 110ml | GBI Labs |
D42-18/D42-6 | Polink-2 Plus HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D68-110 | Polink-2 Plus AP Broad Bulk Kit | 110ml | GBI Labs |
D68-18/D68-6 | Polink-2 Plus AP Broad Kit | 18ml/6ml | GBI Labs |
D13-18/D13-6 | Polink-1 HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D16-110 | Polink-1 HRP Rabbit for AEC Bulk Kit | 110ml | GBI Labs |
D16-18/D16-6 | Polink-1 HRP Rabbit with AEC Kit | 18ml/6ml | GBI Labs |
D19-110 | Polink-1 AP Rabbit Bulk Kit | 110ml | GBI Labs |
D19-18/D19-6 | Polink-1 AP Rabbit with Permanent Red Kit | 18ml/6ml | GBI Labs |
D39-110 | Polink-2 Plus HRP Rabbit for DAB Bulk Kit | 110ml | GBI Labs |
D39-18/D39-6 | Polink-2 Plus HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D40-110 | Polink-2 Plus HRP Rabbit for AEC Bulk Kit | 110ml | GBI Labs |
D40-18/D40-6 | Polink-2 Plus HRP Rabbit with AEC Kit | 18ml/6ml | GBI Labs |
D70-110 | Polink-2 Plus AP Rabbit Bulk Kit | 110ml | GBI Labs |
D70-18/D70-6 | Polink-2 Plus AP Rabbit with Permanent Red Kit | 18ml/6ml | GBI Labs |
D03(2)-110 | Biotin-2nd Ab Rabbit | 110mL | GBI Labs |
D03(2)-18 | Biotin-2nd Ab Rabbit | 18mL | GBI Labs |
D03-110 | SPlink HRP Rabbit Bulk Kit | 110ml | GBI Labs |
D03-18/D03-6 | SPlink HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D03-500 | SPlink HRP Rabbit Bulk Kit | 500mL | GBI Labs |
D34-18/D34-6 | Polink-1 HRP Goat with AEC Kit | 18ml/6ml | GBI Labs |
D61-110 | Polink-1 AP Goat Bulk Kit | 110ml | GBI Labs |
D61-18/D61-6 | Polink-1 AP Goat with Permanent Red Kit | 18ml/6ml | GBI Labs |
D43-110 | Polink-2 Plus HRP Goat for DAB Bulk Kit | 110ml | GBI Labs |
D43-18/D43-6 | Polink-2 Plus HRP Goat with DAB Kit | 18ml/6ml | GBI Labs |
D45-110 | Polink-2 Plus HRP Goat for AEC Bulk Kit | 110ml | GBI Labs |
D45-18/D45-6 | Polink-2 Plus HRP Goat with AEC Kit | 18ml/6ml | GBI Labs |
D66-110 | Polink-2 Plus AP Goat Bulk Kit | 110ml | GBI Labs |
D66-18/D66-6 | Polink-2 Plus AP Goat with Permanent Red Kit | 18ml/6ml | GBI Labs |
D76(2)-18 | Biotin-2nd Ab Goat | 18mL | GBI Labs |
D76-110 | SPlink HRP Goat Bulk Kit | 110ml | GBI Labs |
D76-18/D76-6 | SPlink HRP Goat with DAB Kit | 18ml/6ml | GBI Labs |
D77-18/D77-6 | SPlink HRP Goat AEC Kit | 18ml/6ml | GBI Labs |
D84-18/D84-6 | Polink-2 Plus HRP Chicken IgY with DAB Kit | 18ml/6ml | GBI Labs |
D84-60 | Polink-2 Plus HRP Chicken IgY for DAB Kit | 60ml | GBI Labs |
D92-18/D92-6 | Splink HRP Chicken IgY with DAB Kit | 18ml/6ml | GBI Labs |
D92-60 | Splink HRP Chicken IgY Bulk Kit | 60ml | GBI Labs |
D93-18/D93-6 | Splink HRP Chicken IgY with AEC Kit | 18ml/6ml | GBI Labs |
D85-18/D85-6 | Polink-2 Plus HRP Sheep with DAB Kit | 18ml/6ml | GBI Labs |
D52-18/D52-6 | Klear Mouse HRP with DAB Kit | 18ml/6ml | GBI Labs |
D53-110 | Klear Mouse HRP for AEC Bulk Kit | 110ml | GBI Labs |
D53-18/D53-6 | Klear Mouse HRP with AEC Kit | 18ml/6ml | GBI Labs |
D54-100 | Klear Mouse Blocking Buffer | 100mL | GBI Labs |
D54-18/D54-6 | Klear Mouse Blocking Buffer | 18mL/6mL | GBI Labs |
D55-110 | Polink-1 HRP Mouse-NR for DAB Bulk Kit | 110ml | GBI Labs |
D55-18/D55-6 | Polink-1 HRP Mouse-NR with DAB Kit | 18ml/6ml | GBI Labs |
D34-110 | Polink-1 HRP Goat for AEC Bulk Kit | 110ml | GBI Labs |
D34-18/D34-6 | Polink-1 HRP Goat with AEC Kit | 18ml/6ml | GBI Labs |
D61-110 | Polink-1 AP Goat Bulk Kit | 110ml | GBI Labs |
D61-18/D61-6 | Polink-1 AP Goat with Permanent Red Kit | 18ml/6ml | GBI Labs |
D43-110 | Polink-2 Plus HRP Goat for DAB Bulk Kit | 110ml | GBI Labs |
D43-18/D43-6 | Polink-2 Plus HRP Goat with DAB Kit | 18ml/6ml | GBI Labs |
D45-110 | Polink-2 Plus HRP Goat for AEC Bulk Kit | 110ml | GBI Labs |
D45-18/D45-6 | Polink-2 Plus HRP Goat with AEC Kit | 18ml/6ml | GBI Labs |
D66-110 | Polink-2 Plus AP Goat Bulk Kit | 110ml | GBI Labs |
D66-18/D66-6 | Polink-2 Plus AP Goat with Permanent Red Kit | 18ml/6ml | GBI Labs |
D76-110 | SPlink HRP Goat for DAB Bulk Kit | 110ml | GBI Labs |
D76-18/D76-6 | SPlink HRP Goat with DAB Kit | 18ml/6ml | GBI Labs |
D77-18/D77-6 | SPlink HRP Goat with AEC Kit | 18ml/6ml | GBI Labs |
D84-18/D84-6 | Polink-2 Plus HRP Chicken IgY with DAB Kit | 18ml/6ml | GBI Labs |
DS201B-6 (D63-6) | Polink DS-MR-Hu B1 Kit | 12ml | GBI Labs |
DS201B-60 | Polink DS-MR-Hu B1 Kit | 120ml | GBI Labs |
DS201C-18 | Polink DS-MR-Hu C1 Kit | 36mL | GBI Labs |
DS201C-6 | Polink DS-MR-Hu C1 Kit | 12mL | GBI Labs |
DS201C-60 | Polink DS-MR-Hu C1 Kit | 120mL | GBI Labs |
DS201D-18 | Polink DS-MR-Hu D1 Kit | 36ml | GBI Labs |
DS201D-6 (D20-6F) | Polink DS-MR-Hu D1 Kit | 12ml | GBI Labs |
DS201D-60 | Polink DS-MR-Hu D1 Kit | 120ml | GBI Labs |
DS202A-18 | Polink DS-MR-Hu A2 Kit | 36mL | GBI Labs |
DS202A-6 (D32-6A) | Polink DS-MR-Hu A2 Kit | 12mL | GBI Labs |
DS202A-60 | Polink DS-MR-Hu A2 Kit | 120mL | GBI Labs |
DS202B-18 | Polink DS-MR-Hu B2 Kit | 36ml | GBI Labs |
DS202B-6 (D64-6) | Polink DS-MR-Hu B2 Kit | 12ml | GBI Labs |
DS202B-60 | Polink DS-MR-Hu B2 Kit | 120ml | GBI Labs |
DS202C-18 | Polink DS-MR-Hu C2 Kit | 36ml | GBI Labs |
DS202C-6 | Polink DS-MR-Hu C2 Kit | 12ml | GBI Labs |
DS202C-60 | Polink DS-MR-Hu C2 Kit | 120ml | GBI Labs |
DS202D-18 | Polink DS-MR-Hu D2 Kit | 36ml | GBI Labs |
DS202D-6 (D32-6F) | Polink DS-MR-Hu D2 Kit | 12ml | GBI Labs |
DS202D-60 | Polink DS-MR-Hu D2 Kit | 120ml | GBI Labs |
DS203A-18 | Polink DS-MM-Hu A Kit | 36mL | GBI Labs |
DS203A-6 (D78-6A) | Polink DS-MM-Hu A Kit | 12mL | GBI Labs |
DS203A-60 | Polink DS-MM-Hu A Kit | 120mL | GBI Labs |
DS203B-18 | Polink DS-MM-Hu B Kit | 36ml | GBI Labs |
DS203B-6 (D79-6) | Polink DS-MM-Hu B Kit | 12ml | GBI Labs |
DS203B-60 | Polink DS-MM-Hu B Kit | 120ml | GBI Labs |
DS203C-18 | Polink DS-MM-Hu C Kit | 36mL | GBI Labs |
DS203C-6 | Polink DS-MM-Hu C Kit | 12mL | GBI Labs |
DS203C-60 | Polink DS-MM-Hu C Kit | 120mL | GBI Labs |
DS203D-18 | Polink DS-MM-Hu D Kit | 36ml | GBI Labs |
DS203D-6 (D78-6F) | Polink DS-MM-Hu D Kit | 12ml | GBI Labs |
DS203D-60 | Polink DS-MM-Hu D Kit | 120ml | GBI Labs |
DS204A-18 | Polink DS-RR-Hu/Ms A Kit | 36ml | GBI Labs |
DS204A-6 (D80-6A) | Polink DS-RR-Hu/Ms A Kit | 12ml | GBI Labs |
DS204A-60 | Polink DS-RR-Hu/Ms A Kit | 120ml | GBI Labs |
DS204B-18 | Polink DS-RR-Hu/Ms B Kit | 36ml | GBI Labs |
DS204B-6 (D81-6) | Polink DS-RR-Hu/Ms B Kit | 12ml | GBI Labs |
DS204B-60 | Polink DS-RR-Hu/Ms B Kit | 120ml | GBI Labs |
DS204C-18 | Polink DS-RR-Hu/Ms C Kit | 36mL | GBI Labs |
DS204C-6 | Polink DS-RR-Hu/Ms C Kit | 12mL | GBI Labs |
DS204C-60 | Polink DS-RR-Hu/Ms C Kit | 120mL | GBI Labs |
DS204D-18 | Polink DS-RR-Hu/Ms D Kit | 36ml | GBI Labs |
DS204D-6 (D80-6F) | Polink DS-RR-Hu/Ms D Kit | 12ml | GBI Labs |
DS204D-60 | Polink DS-RR-Hu/Ms D Kit | 120ml | GBI Labs |
DS207A-18 | Polink DS-GM-Hu A Kit | 36ml | GBI Labs |
DS207A-6 | Polink DS-GM-Hu A Kit | 12ml | GBI Labs |
DS207A-60 | Polink DS-GM-Hu A Kit | 120ml | GBI Labs |
DS207B-18 | Polink DS-GM-Hu B Kit | 36ml | GBI Labs |
DS207B-6 | Polink DS-GM-Hu B Kit | 12ml | GBI Labs |
DS207B-60 | Polink DS-GM-Hu B Kit | 120ml | GBI Labs |
DS207C-18 | Polink DS-GM-Hu C Kit | 36ml | GBI Labs |
DS207C-6 | Polink DS-GM-Hu C Kit | 12ml | GBI Labs |
DS207C-60 | Polink DS-GM-Hu C Kit | 120ml | GBI Labs |
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