5-甲基胞苷-5-三磷酸盐是TRILINK公司产品,用于干细胞研究用,5-methyl-ctp Synonyms] 5-methyl-dCTP 5-methyldeoxycytidine triphosphate CPD-1094 5-methyl deoxycytidine-5'-triphosphate 5-methyl-2'-deoxycytidine-5'-triphosphate cytidine 5'-(tetrahydrogen triphosphate), 2'-deoxy-5-methyl- [[[5-[(4-amino-5-methyl-2-oxo-1H-pyrimidin-1-yl)]-3-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxyphosphonic acid
[Structure] [ Properties Computed from Structure]
Molecular Weight | 481.183503 [g/mol] | Molecular Formula | C10H18N3O13P3 | XLogP | -5.9 | H-Bond Donor | 6 | H-Bond Acceptor | 14 | Rotatable Bond Count | 8 | Tautomer Count | 3 | Exact Mass | 481.005247 | MonoIsotopic Mass | 481.005247 | Topological Polar Surface Area | 248 | Heavy Atom Count | 29 | Formal Charge | 0 | Complexity | 868 | Isotope Atom Count | 0 | Defined Atom StereoCenter Count | 0 | Undefined Atom StereoCenter Count | 3 | Defined Bond StereoCenter Count | 0 | Undefined Bond StereoCenter Count | 0 | Covalently-Bonded Unit Count | 1 | Description 5-Methyl-dCTP is widely used for construction of cDNA libraries | Incorporation of 5-Methyl-dCTP M-MuLV Reverse Transcriptase Klenow Fragment of DNA Polymerase I Sequenase DNA Polymerase (Taq Polymerase, Vent) * Incorporation of Hg-dCTP DNA Polymerase I References to 5-Methyl-dCTP Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584. Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 (3):681. Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research 6:369. * Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 (5):1081. Reference to Hg-dCTP Banfalvi et al. (1995) Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 (5):445. | N-1014-1 | 5-Methylcytidine-5'-TP | 1umole | 1317.5 | N-1014-10 | 5-Methylcytidine-5'-TP | 10umoles | 10625 | N-1014-5 | 5-Methylcytidine-5'-TP | 5umoles | 6205 | Reference(s) | Kariko K, Muramatsu H, Ludwig J, Weissman D. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. (2011) Nucleic Acids Research. | | | | | | Kormann M, Hasenpusch G, Aneja M, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. (2011) Nature Biotechnology 29:154–157. | | | | | | Anderson, B., Muramatsu, H., Nallagatla, S.R., Bevilacqua, P.C., Sansing, L.H., Weissman, D. & Kariko, K. Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation (2010) Nucleic Acids Research, 38(17): 5884-5892. | | | | | | Warren et al., Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell (2010), doi:10.1016/j.stem.2010.08.012. | | | | | | Kariko K, Muramatsu H, Welsh F, et al. Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. (2008) Molecular Therapy (16)11: 1833-1840. | | | | | | Kariko, K., Buckstein, M., Ni, H. & Weissman, D. Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA (2005). Immunity, 23(2), 165-175. | | | | | | Lefmann M, et al. Novel Mass Spectrometry-based tool for genotypic identification of mycobacteria. (2004) Journal of Clinical Microbiology, 42(1): 339-346. | | | | | | Hartmer R, Storm N, Boecker S, Rodi CP, Hillenkamp F, Jurinke C, van den Boom D. RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. (2003) Nucleic Acids Res., 31(9): e47. | | | | | | Nguyen A, Zhao C, Dorris D, Mazumder A. Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. (2002) BMC Biotechnology, 2(1): 14. | | | | | | Van Rompay AR, Norda A, Linden K, Johansson M, Karlsson A. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. (2001) Mol Pharmacol., 59(5): 1181-6. | | | | | | |