LenioBio GmbH是一家专注于蛋白质表达解决方案的生物技术公司。LenioBio总部位于德国杜塞尔多夫,由商业资深人士和蛋白质表达专家于2016年创立。2018年三月,LenioBio签署了陶氏益农的许可协议,以推出自己专有的无细胞蛋白质表达技术下LenioBio品牌爱丽丝®。LenioBio目前正在进一步开发该技术,通过智能设计进行创新,以利用自然的力量来加速合成生物学。历史和背后的爱丽丝技术的特点®在技术部分描述。
LenioBio AL0103500说明书
“爱丽丝®是一个*的真核无细胞系统,可以生成一个的3毫克/毫升在批处理模式的蛋白质,与市场上的其它真核无细胞系统,它仅产生0.1毫克/毫升或更小进行比较。即使是原核细胞无系统,通常只能处理易于表达的蛋白质,只能以分批模式产生1 mg / mL的蛋白质。“
Manual for Protein Expression with ALiCE®
套件组件
细胞裂解物来自植物细胞
保质期
12个月
贮存温度
-70°C至-80°C
活动验证
至少3毫克的表达/以下爱丽丝的手动时毫升增强黄色荧光蛋白的®
Certificate of Analysis
Product Name: ALiCE® Maxi Kit
产品名称:Alice Maxi Kit
Date: 05.10.2018
产品编号:AL 0103500
包装编号:#2018-AL 01-781
届满日期:06/2019
储存温度:-80℃
分析和结果
Prod. Number | Component Description | Lot Number | Assay | QC Result |
LA0103500 | ALiCE® Reaction mix, 50 µL | #2018-A004 | A 50 µL reaction is performed with 500 ng (2 µL) of pALiCE01 plasmid and 48 µL of Reaction mix in a 96 half well plate with lid. Active eYFP protein mass is determined by fluorometric comparison with a fluorescein standard after 48 hours. eYFP concentration must be between 3.0 – 3.5 mg/mL. Reaction conditions: Temperature: 25 °C Humidity: 75 % Shaking frequency: 500 rpm Shaker: Kuhner LT-X, shaking diameter: 25 mm 96 half well plates: Greiner bio-one, Art. No. 675086 Date: 13.06.2018 | pass |
PA0101050 | Plasmid pALiCE01, 50 µL | #2018-PA01-281 | Concentration of the plasmid is determined by photometric concentration measurement. Concentration must be between 245 – 255 ng/µL. Plasmid sequence is controlled by sequencing analysis. Date: 09.08.2018 | pass |
PA0102050 | Plasmid pALiCE02, 50 µL | #2018-PA02-124 | Concentration of the plasmid is determined by photometric concentration measurement. Concentration must be between 245 – 255 ng/µL. Plasmid sequence is controlled by sequencing analysis. | pass |
ALiCE® Cell-Free Protein Expression Maxi Kit | 6 x 500µl | € 3470 |
ALiCE® Cell-Free Protein Expression Midi Kit | 6 x 200µl | € 1520 |
ALiCE® Cell-Free Protein Expression Mini Kit | 6 x 50µl | € 395 |
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