世界转染试剂汇总

更新时间：2017-02-14

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简要描述：

世界上有很多转染试剂，到底你该选用哪种？

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产品介绍

promega

产品名称

包装

目录号

价格(元)

FuGENE® 6 Transfection Reagent

1ml

E2691

3976

FuGENE® HD Transfection Reagent

5 × 1ml

E2312

33264

TransFastTM Transfection Reagent

1.2mg

E2431

2247

FuGENE® 6 Transfection Reagent

5 × 1ml

E2692

16190

FuGENE® 6 Transfection Reagent

0.5ml

E2693

2294

FuGENE® HD Transfection Reagent

1ml

E2311

6720

origene

多种转染试剂满足您的特定的cDNA和shRNA转染需求。

Cell Types	Examples	Recommended Reagents
Commonly used established cell lines	Hela, MCF 7, K562, Jurkat, 3T3, COS	TurboFectin 8.0
Most established cell lines and primary cells	Above, plus MEF, HUVEC, SMC, lymphocytes, etc.	Magnetofection PolyMag Magnetofection CombiMag
Primary neurons and neuronal cell lines	Hippocampal neurons and neuronal cell lines PC-12, B95, C6, N2A or SH-5YSY, etc.	Magnetofection NeuroMag
Virus infection of non-permissive cells		Magnetofection ViroMag
HEK293T, CHO, 293S, etc		MegaTran 1.0
For siRNA transfection	HEK293T	siTran 1.0

TurboFectin 8.0^TM是针对核酸转染真核细胞所优化的一种高效率的新型转染试剂，由配方的脂质体和组蛋白混合物，溶在80%的酒精中。适用于在各种不同构建类型的细胞中转染TrueClone®/TrueORF®克隆 (基因过表达) 以及 HuSH-29® (用于基因沉默的shRNA)。
- 性能优越: 与FuGENE®6 相媲美的高转染效率和低毒性
- 应用广泛: 使用TurboFectin 8.0 已经成功转染超过100种细胞系及原代细胞类型
- 价格优惠: 比FuGENE^®价格便宜
Magnetofection ^TM是一种新型, 简易，高效的转染方法。利用磁力将含有磁珠的目的基因载体，吸向靶细胞。
- 可用于更多种细胞，更加适用于难转染的细胞。包括原代细胞和神经细胞
- 与常规转染方法相比，转染效率极大提高。
- 在较短的孵育过程内，拥有比常规转染方法高上千倍的转染效率
了解更多春季促销
MegaTran 1.0 是一种非脂质体聚合物，适用于大量DNA和shRNA转染及大规模蛋白质制备。
适用细胞系：CHO，HEK293，293F suspension cells，Hela，COS-7，NIH3T3，MEF，Sf9，U2OS等。
- 高效，低毒。
- 提高瞬时转染后蛋白的表达量。
- 价格非常优惠。

INVITROGEN

Transfection Reagent	Key Features & Applications of Reagent
Lipofectamine™ LTX Transfection Reagent	High transfection efficiency and significantly lower toxicity levels for a wide range of cell lines Significantly improved transfection performance in a number of primary and hard-to-transfect cell lines Optimized protocols for over 30 different cell lines are available so much less time is needed for evaluation and optimization
FreeStyle™ MAX Transfection Reagent	Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells Used for a large-scale transient protein production with milligrams of protein yield
Lipofectamine™ RNAiMAX Transfection Reagent	Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent Compatibility with a broad range of cell types providing the most versatile approach to all of your gene silencing experiments
Lipofectamine™ 2000 Transfection Reagent	Achieves highest efficiency and expression results for plasmid or RNAi transfections Works effectively with many cell types (adherent or suspension) Easy and fast protocol – no need for wash steps before or after transfection Complexes can be added to cells growing in serum-containing media Ideal if transfecting at confluencies of 90% or greater (minimizes cytotoxicity following transfection)
Lipofectamine™ 2000 CD Transfection Reagent	Same performance as Lipofectamine™ 2000, certified animal-origin free (“CD” = chemically defined)
Oligofectamine™ Transfection Reagent	Transfection of antisense oligonucleotides
293fectin™ Transfection Reagent	Used for transient protein production in combination with the FreeStyle™ 293 Expression System Optimized for suspension FreeStyle™ 293-F cells
Cellfectin® Transfection Reagent	Optimal transfection of insect cells, including S2, Sf9, Sf21 and High Five™ cells
DMRIE-C Transfection Reagent	Transfection of suspension cells, including CHO, lymphoid and Jurkat cell lines.
Lipofectamine™ Transfection Reagent	First generation reagent for plasmid DNA transfections In most cases, Lipofectamine™ 2000 provides better performance than Lipofectamine™ Transfection Reagent
Lipofectin® Transfection Reagent	First generation reagent for plasmid DNA transfections In most cases, Lipofectamine™ 2000 provides better performance than Lipofectin® Transfection Reagent
Optifect™ Transfection Reagent	Broad use reagent designed for low confluency applications (<70% confluent at the time of transfection) Useful for cell lines that are sensitive to transfection reagents

Plasmid DNA Transfection

To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents to mediate efficient delivery into the cell's nucleus. This process is essential for subsequent protein expression of the gene of interest. Plasmids are small circular DNA molecules that naturally occur in bacteria, and are actually used by the bacteria to transfer genetic information. The mechanism of adding a DNA Plasmid into a mammalian cell is known as plasmid transfection.

Invitrogen offers a wide range of transfection reagents for your specific needs. For cell types specific protocols, please refer to Transfection Selection Tool.

Order:
- Plasmid Transfection Reagents
- Electroporation Instruments & Reagents

Featured Product - Lipofectamine™ LTX with PLUS™ Reagent

Our latest and most efficient Lipofectamine™ product. The best choice for transfecting plasmid DNA into standard and other difficult to transfect cell types; highest transfection efficiency and viability with superb expression for all cell types.

High transfection efficiency and high gene expression with minimal cytotoxicity in a broad range of cells
Significantly improved transfection performance in a number of primary and disease-relevant cell types
No washes or media changes required - just set up and go!

In 90% of the cell lines that we have tested, transfection performance is enhanced by using Lipofectamine™ LTX and PLUS™ Reagent together.